Carboxy-H2DCFDA (general oxidative stress indicator)
Carboxy-H2DCFDA (general oxidative stress indicator)
인용 및 참조 문헌 (95)
Invitrogen™

Carboxy-H2DCFDA (general oxidative stress indicator)

6-carboxy-2',7'-dichlorodihydrofluorescein diacetate는 환원된 아세틸화 된 fluorescein으로 세포 내 활성 산화종(ROS)의 표지자로 사용됩니다. 이 비형광 분자는 세포 간 esterase로 아세테이트기가 제거되고자세히 알아보기
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카탈로그 번호수량
C40025 mg
카탈로그 번호 C400
제품 가격(KRW)
425,000
キャンペーン価格
Ends: 31-Dec-2025
472,000
할인액 47,000 (10%)
Each
카트에 추가하기
수량:
25 mg
제품 가격(KRW)
425,000
キャンペーン価格
Ends: 31-Dec-2025
472,000
할인액 47,000 (10%)
Each
카트에 추가하기
6-carboxy-2',7'-dichlorodihydrofluorescein diacetate는 환원된 아세틸화 된 fluorescein으로 세포 내 활성 산화종(ROS)의 표지자로 사용됩니다. 이 비형광 분자는 세포 간 esterase로 아세테이트기가 제거되고 세포 내 산화(ROS의 활성)가 발생하면 녹색 형광 형태로 쉽게 전환됩니다. 자사는 생리적 pH에서 음전하 2개를 가진 carboxylated H2DCFDA 유사체(carboxy-H2DCFDA, C400)와 di(acetoxymethyl ester) (C2938)를 제공합니다. 세포 간 esterase와 산화로 아세테이트기와 에스테르기가 절단되면 두 개의 analogs는 음전하를 띠는 carboxydichlorofluorescein (C368) 밖으로 방출되지 않고 세포 내에 유지하게 합니다.

ROS 표지자 사양:
• Ex/Em: ∼492–495/517–527 nm
• 제품은 공기에 민감하기 때문에 건조 아르곤이나 질소에 보관해야 합니다.• DMSO, DMF, 또는 에탄올에 녹여 사용할 수 있습니다.• 이는 세포 투과성이 있습니다(cell loading protocol을 문헌에서 찾아볼 수 있습니다).
• 형광 모니터링은 flow cytometer, fluorometer, microplate reader, 형광 현미경을 사용하거나 fluorescein에 적합한 여기원(excitation sources)과 필터를 사용해 실시할 수 있습니다.

더 많은 ROS 표지자 찾기
본사는 singlet oxygen, superoxide, hydroxyl radical , 다양한 peroxide 및 hydroperoxide 등의 활성산소종(ROS) 생성에 대한 많은 Molecular Probes™ 제품을 제공합니다. 이 제품에 대해 더 많은 정보를 원하신다면 Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook을 참조하십시오.

본 제품은 연구용으로만 사용할 수 있습니다. 사람이나 동물의 치료 또는 진단용으로 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
수량25 mg
제품 유형ROS Indicator
Unit SizeEach

자주 묻는 질문(FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (95)

인용 및 참조 문헌
Abstract
N-acetylcysteine augments the cellular redox changes and cytotoxic activity of internalized mycobacterium bovis in human bladder cancer cells.
Authors:Pook SH, Esuvaranathan K, Mahendran R
Journal:J Urol
PubMed ID:12131368
PURPOSE: We determined whether changes in cellular reactive oxygen species correlated with mycobacteria internalization and bladder cancer cell death. MATERIALS AND METHODS: Reactive oxygen species and thiols in RT112 and MGH bladder cancer cells were determined using the fluorescence probes 5-(and 6)-carboxy-2', 7' dichlorodihydrofluorescein diacetate and monobromobimane. Superoxide and nitrite ... More
The sulphonylurea glibenclamide inhibits multidrug resistance protein (MRP1) activity in human lung cancer cells.
Authors:Payen L, Delugin L, Courtois A, Trinquart Y, Guillouzo A, Fardel O
Journal:Br J Pharmacol
PubMed ID:11159731
'1. Glibenclamide, a sulphonylurea widely used for the treatment of non-insulin-dependent diabetes mellitus, has been shown to inhibit the activities of various ATP-binding cassette (ABC) transporters. In the present study, its effects towards multidrug resistance protein 1 (MRP1), an ABC efflux pump conferring multidrug resistance and handling organic anions, were ... More
Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae.
Authors:Shiflett SL, Ward DM, Huynh D, Vaughn MB, Simmons JC, Kaplan J
Journal:J Biol Chem
PubMed ID:14701806
'We identified VTA1 in a screen for mutations that result in altered vacuole morphology. Deletion of VTA1 resulted in delayed trafficking of the lipophilic dye FM4-64 to the vacuole and altered vacuolar morphology when cells were exposed to the dye 5-(and 6)-carboxy-2'',7''-dichlorofluorescein diacetate (CDCFDA). Deletion of class E vacuolar protein ... More
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes.
Authors:Diaz G, Liu S, Isola R, Diana A, Falchi AM
Journal:Histochem Cell Biol
PubMed ID:14574587
'Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-L-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2'',7''-dichlorodihydrofluorescein diacetate di-acetomethyl ester ... More
The use of fluorescent probes to assess oxidative processes in isolated-perfused rat heart tissue.
Authors:Kehrer JP, Paraidathathu T
Journal:Free Radic Res Commun
PubMed ID:1505782
'The formation of reactive oxygen species (ROS) in intact heart tissue has been assessed by direct ESR measurements, and indirectly by the formation of characteristic tissue products and the protective effects of various antioxidants. The development of lipid soluble esters of compounds which can be trapped intracellularly after hydrolysis, and ... More
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