MultiShot™ TOP10 Chemically Competent E. coli

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.자세히 알아보기

카탈로그 번호	수량
C40005 C400-05으로도 사용됨	5 x 96-well Plate (15 μL/Well)

카탈로그 번호 C40005

C400-05으로도 사용됨

제품 가격(KRW)

5 x 96-well Plate (15 μL/Well)

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains. Packaged in five 96-well microtiter plates (15 μL aliquots) to simplify high-throughput workflows and provide transformation efficiencies of >1 x 10⁸ cfu/μg control plasmid DNA, Multishot TOP10 is a versatile strain that can be used in multiple routine cloning applications.

Benefits of MultiShot TOP10 competent cells
• TOP10 strain is ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design is convenient for automated high-throughput cloning
• mcrA mutation supports efficient transfection of methylated DNA
• lacZΔM15 allele permits rapid blue/white screening of transfectants
• endA1 mutation reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation helps reduce unwanted recombination

Easy to use for high-throughput transformations
MultiShot TOP10 Chemically Competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Using our TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that fits your needs
DH strains are available in chemically competent and electrocompetent cell formats.
For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.
Email us for more information on custom formatting options.

For Research Use Only. Not for use in diagnostic procedures.

항생제 내성 박테리아Yes (Streptomycin)

블루/화이트 스크리닝Yes (lacZΔM15)

메틸화 DNA 클로닝Yes (mcrA)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

에프에피솜 포함No

고처리량 호환성High

플라스미드 품질 개선Yes (endA1)

플라스미드High Copy Plasmid

비메틸화 DNA 준비No

제품라인MultiShot

제품 유형Chemically Competent Cells

수량5 x 96-well Plate (15 μL/Well)

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

형질전환 효율 수준Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)

형식96-well Plate

종E. coli (K12)

Unit SizeEach

구성 및 보관

• MultiShot™ TOP10 Chemically Competent E. coli (5 x 96-well plates at 15 μL per well)
Store Competent Cells at –80°C.

• pUC19 DNA (1 x 50 μL)
Store pUC19 DNA at –20°C.

• S.O.C. medium (5 x 15 mL/bottle)
Store S.O.C. Medium at 4°C or at room temperature

자주 묻는 질문(FAQ)

What are the differences between the MultiShot StripWell, MultiShot FlexPlate, and MultiShot formats of E. coli cells?

The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.

The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.

The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.