CM-H2DCFDA (General Oxidative Stress Indicator)

인용 및 참조 문헌 (115)

Invitrogen™

CM-H2DCFDA (General Oxidative Stress Indicator)

CM-H2DCFDA는 H2DCFDA의 chloromethyl derivative로 세포 내 활성산소(ROS) 표지자로 유용합니다. 이 표지자는 H2DCFDA보다 살아있는 세포에서 유지력이 좋습니다. CM-H2DCFDA는 세포에 수동확산되는데 여기에서자세히 알아보기

카탈로그 번호	수량
C6827	20 x 50 μg

카탈로그 번호 C6827

제품 가격(KRW)

642,000

キャンペーン価格

Ends: 31-Mar-2026

713,000

할인액 71,000 (10%)

카트에 추가하기

20 x 50 μg

제품 가격(KRW)

642,000

キャンペーン価格

Ends: 31-Mar-2026

713,000

할인액 71,000 (10%)

카트에 추가하기

CM-H₂DCFDA는 H₂DCFDA의 chloromethyl derivative로 세포 내 활성산소(ROS) 표지자로 유용합니다. 이 표지자는 H₂DCFDA보다 살아있는 세포에서 유지력이 좋습니다. CM-H₂DCFDA는 세포에 수동확산되는데 여기에서 acetate groups이 intracellular esterases로 절단되고 thiol-reactive chloromethyl group은 intracellular glutathione 및 다른 thiols과 반응합니다. 그리고 산화 반응으로 세포 안에 갇혀 있는 형광 부가생성물을 회수하기 때문에 장기간 연구에 사용하기 편리합니다.

ROS 표지자 명세:

• Ex/Em: ∼492–495/517–527 nm
• 제품은 공기에 민감하기 때문에 드라이 아르곤이나 질소에 보관해야 합니다.
• DMSO, DMF, 또는 에탄올에 녹여 사용할 수 있습니다.
• 이는 세포 투과성이 있습니다(세포 부하 프로토콜을 문헌에서 찾아볼 수 있습니다.)
• 형광 모니터링은 유세포 분석기나 마이크로플레이트 리더기, 형광 현미경을 사용하거나 fluorescein에 적합한 여기원과 필터를 사용해 실시할 수 있습니다.

더 많은 ROS 표지자 찾기
본사는 단일 활성산호, 수퍼옥사이드, 히드록실라디칼, 다양한 퍼옥사이드, 하이드로퍼옥사이드 등 활성산호(ROX) 생성에 대한 많은 Molecular Probes™ 제품을 제공합니다. 이 제품에 대해 더 많은 정보를 원하신다면 Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook를 참조하세요.

본 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.

For Research Use Only. Not for use in diagnostic procedures.

수량20 x 50 μg

제품 유형ROS Indicator

Unit SizeEach

자주 묻는 질문(FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H₂DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (115)

인용 및 참조 문헌

Abstract

Authors:

Journal:

PubMed ID:18258751

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.

Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K

Journal:Am J Physiol Renal Physiol

PubMed ID:16159901

'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More

The yeast prion Ure2p native-like assemblies are toxic to mammalian cells regardless of their aggregation state.

Authors:Pieri L, Bucciantini M, Nosi D, Formigli L, Savistchenko J, Melki R, Stefani M

Journal:J Biol Chem

PubMed ID:16571726

'The yeast prion Ure2p assembles in vitro into oligomers and fibrils retaining the alpha-helix content and binding properties of the soluble protein. Here we show that the different forms of Ure2p native-like assemblies (dimers, oligomers, and fibrils) are similarly toxic to murine H-END cells when added to the culture medium. ... More

Role of extracellular signal-regulated protein kinase in neuronal cell death induced by glutathione depletion in neuron/glia mesencephalic cultures.

Authors:de Bernardo S, Canals S, Casarejos MJ, Solano RM, Menendez J, Mena MA

Journal:J Neurochem

PubMed ID:15485497

'To date, glutathione (GSH) depletion is the earliest biochemical alteration shown in brains of Parkinson''s disease patients, but the role of GSH in dopamine cell survival is debated. In this study we show that GSH depletion, produced with GSH synthesis inhibitor, L-buthionine-(S,R)-sulfoximine (BSO), induces selectively neuronal cell death in neuron/glia, ... More

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