Tetrathionate Broth Base (Dehydrated)

Selectively enrich and isolate Salmonella typhi and other salmonellae from feces and sewage with Thermo Scientific™ Oxoid™ Tetrathionate Broth Base (Dehydrated). Organisms which reduce tetrathionate, such a salmonellae, flourish in the medium while many fecal organisms are inhibited¹.

Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract. Salmonella bacteria typically live in animal and human intestines and are shed through feces. Humans become infected most frequently through contaminated water or food.

Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from feces, sewage, etc.

The combination of thiosulphate and tetrathionate render selectivity to the medium, suppressing commensal coliform organisms. Organisms which possess the enzyme tetrathionate reductase grow in the medium. Salmonella and Proteus species possess the enzyme; Escherichia coli and shigellae do not. Members of Proteus group reduce tetrathionate and may consequently impair the value of this medium for the isolation of salmonellae; this disadvantage of the medium is largely overcome by the addition of 40μg of novobiocin to each milliliter of the incomplete medium before the addition of iodine^2,3.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.