Staphylococcus Medium No. 110 (Dehydrated)

Selectively isolate and differentiate pathogenic staphylococci based on salt tolerance, pigmentation, mannitol fermentation and gelatin liquefaction with Thermo Scientific™ Oxoid™ Staphylococcus Medium No.110 (Dehydrated). Pathogenic staphylococci (coagulase-positive) grow on the high-salt mannitol medium to form orange colonies which give positive reactions for acid production and gelatin liquefaction.

Use Staphylococcus Medium No.110 for selective isolation and differentiation of pathogenic staphylococci (Chapman^1,2) based on salt tolerance, pigmentation, mannitol fermentation, and gelatin liquefaction.

The Staphylococcus Medium No.110 is formulated according to the APHA³ and AOAC⁴ specifications and can be used with Egg Yolk Emulsion (5% v/v SR0047) to study the egg yolk reactions.

Stone⁵ suggested that the gelatinase activity in the medium was indicative of food poisoning strains but Chapman et al.⁶ reported that typical food poisoning staphylococci should also produce an orange pigment, be hemolytic, be coagulase-positive, and ferment mannitol. Chapman⁷ showed that incubation at 30°C produced deeper pigmentation and no interference with the Stone reaction or with acid production from mannitol - both latter being about as intense as at 35°C.

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