ChargeSwitch™ Plasmid Yeast Mini Kit

Invitrogen™

ChargeSwitch™ Plasmid Yeast Mini Kit

Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng• Increased transformation efficiency• Reagents that avoid PCR자세히 알아보기

카탈로그 번호	수량
CS10203	50 Preps

카탈로그 번호 CS10203

제품 가격(KRW)

409,000

온라인 행사

Ends: 31-Dec-2025

454,000

할인액 45,000 (10%)

카트에 추가하기

50 Preps

제품 가격(KRW)

409,000

온라인 행사

Ends: 31-Dec-2025

454,000

할인액 45,000 (10%)

카트에 추가하기

Sample size 1 ml S. cerevisiae culture; Typical yield up to 25 ng

• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance

The ChargeSwitch™ Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 ml of Saccharomyces cerevisiae grown in selective minimal media. This kit typically yields up to 25 ng of plasmid DNA. Data generated from transformation and PCR experiments using ChargeSwitch™ purified DNA shows improved performance versus DNA purified by other methods (Figure 1). This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

For Research Use Only. Not for use in diagnostic procedures.

최종 제품 유형Plasmid DNA

용도(애플리케이션)PCR, Cloning, Southern Blotting, Sequencing, Transformation

그린 기능Less hazardous, Fewer resources used and less waste

고처리량 호환성Not High-throughput Compatible (Manual)

반응 수50 Preps

프렙 스케일< 100 μg (Small-Scale) Plasmid DNA

제품라인ChargeSwitch

제품 유형Plasmid Yeast Mini Kit

수량50 Preps

샘플 종류Yeast

스케일Mini

배송 조건Room Temperature

타겟Plasmid DNA

형식Kit

Isolation TechnologyChargeSwitch Chemistry

Unit SizeEach

구성 및 보관

All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Resuspension Buffer (R4)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

자주 묻는 질문(FAQ)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.