ChargeSwitch™ Forensic DNA Purification Kit
ChargeSwitch™ Forensic DNA Purification Kit
Invitrogen™

ChargeSwitch™ Forensic DNA Purification Kit

Highest sensitivity for poor or environmentally degraded samples• Fully validated for forensic Single Tandem Repeat (STR) analysis• Optimized for rapid자세히 알아보기
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카탈로그 번호반응 수
CS11200100 Preps
CS1120010
CS11200-10으로도 사용됨
960 Preps
카탈로그 번호 CS11200
제품 가격(KRW)
636,000
Exklusiv online
Ends: 31-Dec-2025
706,000
할인액 70,000 (10%)
Each
카트에 추가하기
반응 수:
100 Preps
제품 가격(KRW)
636,000
Exklusiv online
Ends: 31-Dec-2025
706,000
할인액 70,000 (10%)
Each
카트에 추가하기

Highest sensitivity for poor or environmentally degraded samples

• Fully validated for forensic Single Tandem Repeat (STR) analysis
• Optimized for rapid manual or fully automated sample processing

The ChargeSwitch™ Forensic DNA Purification Kit affords the highest sensitivity and robustness. This kit is designed to accommodate all forensic crime scene sample types for STR profiling. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Forensic
고처리량 호환성High-throughput Compatible
반응 수100 Preps
제품라인ChargeSwitch
제품 유형Forensic DNA Purification Kit
수량100 Preps
샘플 종류Forensic Sample
배송 조건Room Temperature
타겟Genomic DNA
테스트 시간15 min.
형식Kit
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
구성 및 보관

All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.

Components:
• ChargeSwitch™ Lysis Buffer (L13)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

자주 묻는 질문(FAQ)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

Do you offer any kits for forensic DNA purification?

Yes, we offer our ChargeSwitch Forensic DNA Purification Kit. Please read more about DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/forensic-dna-extraction.html).

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.