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인용 및 참조 문헌 (1)
Invitrogen™
ChargeSwitch™ gDNA Mini Tissue Kit
The ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| CS11204 | 25 Preps |
카탈로그 번호 CS11204
제품 가격(KRW)
345,000
Online offer
Ends: 31-Mar-2026
405,000할인액 60,000 (15%)
Each
수량:
25 Preps
제품 가격(KRW)
345,000
Online offer
Ends: 31-Mar-2026
405,000할인액 60,000 (15%)
Each
The ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
High Quality, High Yield Plasmid DNA
The ChargeSwitch™ gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 μg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.
Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch™ gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
Improved Performance in Demanding Downstream Applications
The ChargeSwitch™ gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.
For Research Use Only. Not intended for diagnostic procedures.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
High Quality, High Yield Plasmid DNA
The ChargeSwitch™ gDNA Mini Tissue Kit allows purification of genomic DNA from 10-25 mg quantities of tissue typically in less than 15 minutes following sample preparation and lysis. ChargeSwitch™ gDNA Mini Tissue Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds to yield up to 30 μg of gDNA from 0.5 cm mouse tail tips or approximately 25 mg of tissue. For spleen tissue, reduce tissue size to 10 mg.
Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch™ gDNA Mini Tissue Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
Improved Performance in Demanding Downstream Applications
The ChargeSwitch™ gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.
For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)PCR, Southern Blotting, Sequencing, Cloning
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인ChargeSwitch
제품 유형gDNA Mini Tissue Kit
수량25 Preps
샘플 종류Tissue
스케일Mini
배송 조건Room Temperature
타겟Genomic DNA
테스트 시간15 min.
형식Kit
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
구성 및 보관
All components of the ChargeSwitch™ gDNA Tissue Kits are shipped at room temperature. Upon receipt, store the Proteinase K and RNase A at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.
Components:
• ChargeSwitch™ Lysis Buffer (L13)
• ChargeSwitch™ Lysis Buffer (L15)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
자주 묻는 질문(FAQ)
I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?
What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?
How does ChargeSwitch technology work?
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
Murine models of acute neuronopathic Gaucher disease.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17954912
'Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the glucosidase, beta, acid (GBA) gene that encodes the lysosomal enzyme glucosylceramidase (GCase). GCase deficiency leads to characteristic visceral pathology and, in some patients, lethal neurological manifestations. Here, we report the generation of mouse models with