ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch™ PCR Clean-Up Kit
Invitrogen™

ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts자세히 알아보기
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카탈로그 번호반응 수
CS1200010960 Preps
CS12000100 Preps
카탈로그 번호 CS1200010
제품 가격(KRW)
3,104,000
Exklusiv online
Ends: 31-Dec-2025
3,651,000
할인액 547,000 (15%)
Each
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반응 수:
960 Preps
제품 가격(KRW)
3,104,000
Exklusiv online
Ends: 31-Dec-2025
3,651,000
할인액 547,000 (15%)
Each
카트에 추가하기

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

• Sample size: 25–50 μL
• High recovery of DNA fragments
• Rapid, one tube, 5 minute protocol

For Research Use Only. Not for use in diagnostic procedures.
사양
형식Kit
고처리량 호환성High-throughput Compatible
분리 기술ChargeSwitch™ Chemistry
반응 수960 Preps
제품라인ChargeSwitch
제품 유형PCR Clean-Up Kit
수량960 Preps
실행 시간5 min
샘플 종류PCR Products
배송 조건Room Temperature
타겟DNA from Enzymatic Reactions (e.g. PCR)
용도(애플리케이션)Next-Generation Sequencing (NGS), Cloning, Standard PCR
Unit SizeEach
구성 및 보관

The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

자주 묻는 질문(FAQ)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.