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Invitrogen™
Chargeswitch™ Pro Filter Plasmid Midi Kit
The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| CS31104 CS31104 으로도 사용됨 | 25 preps |
카탈로그 번호 CS31104
CS31104 으로도 사용됨
제품 가격(KRW)
1,521,000
Online offer
Ends: 31-Mar-2026
1,854,000할인액 333,000 (18%)
Each
수량:
25 preps
제품 가격(KRW)
1,521,000
Online offer
Ends: 31-Mar-2026
1,854,000할인액 333,000 (18%)
Each
The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.
To accomplish this, the ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)
How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.
For Research Use Only. Not for use in diagnostic procedures.
사양
컬럼 유형Spin Column
용리량0.5 to 1 mL
최종 제품 유형Plasmid DNA
용도(애플리케이션)Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
고처리량 호환성Not High-throughput Compatible (Manual)
포함Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
프렙 스케일100-200 μg (Medium-Scale) Plasmid DNA
제품라인ChargeSwitch
제품 유형Pro Filter Plasmid Midi Kit
순도Transfection Grade
수량25 preps
샘플 종류Bacterial Culture
스케일Midi
유통 기한Guaranteed stable for 6 months when properly stored.
배송 조건Room Temperature
시작 물질 양25 ml
시스템 유형ChargeSwitch™
타겟Plasmid DNA
테스트 시간30 min.
수율200 μg
형식Spin Column
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
구성 및 보관
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.