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인용 및 참조 문헌 (18)
Invitrogen™
DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| D12052 | 1 mg |
카탈로그 번호 D12052
제품 가격(KRW)
522,000
Online offer
Ends: 31-Dec-2025
614,000할인액 92,000 (15%)
Each
수량:
1 mg
제품 가격(KRW)
522,000
Online offer
Ends: 31-Dec-2025
614,000할인액 92,000 (15%)
Each
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent. This quenching of the signal is caused by the close proximity of the dyes on the intact substrate. The enzyme-driven hydrolysis of the substrate results in separation of the dye results in separation of the dye molecules from one another and the fluorescence signal increases.
For Research Use Only. Not for use in diagnostic procedures.
사양
제품라인DQ
수량1 mg
배송 조건Room Temperature
기질Protease Substrate
검출 방법Fluorescence
형태Lyophilized
Substrate PropertiesProtein-Based Substrate
Target EnzymeMetalloproteinase
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.
인용 및 참조 문헌 (18)
인용 및 참조 문헌
Abstract
Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.
Journal:Exp Cell Res
PubMed ID:12581740
'Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using specific cathepsin
Matrix metalloproteinase activity in urine of patients with renal cell carcinoma leads to degradation of extracellular matrix proteins: possible use as a screening assay.
Journal:J Urol
PubMed ID:12629409
'PURPOSE: Localized renal cell carcinoma is usually curable by nephrectomy. However, a large fraction of patients already present with metastatic disease, which results in a poor outcome. Currently no clinically relevant screening assay is available to detect early stage renal cell carcinoma. We investigated whether urinary extracellular matrix (ECM) proteins
Imaging proteolysis by living human glioma cells.
Journal:Biol Chem
PubMed ID:11517931
'Degradation of basement membrane is an essential step for tumor invasion. In order to study degradation in real time as well as localize the site of proteolysis, we have established an assay with living human cancer cells in which we image cleavage of quenched-fluorescent basement membrane type IV collagen (DQ-collagen
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Functional imaging of proteolysis: stromal and inflammatory cells increase tumor proteolysis.
Journal:Mol Imaging
PubMed ID:14649059
'The underlying basement membrane is degraded during progression of breast and colon carcinoma. Thus, we imaged degradation of a quenched fluorescent derivative of basement membrane type IV collagen (DQ-collagen IV) by living human breast and colon tumor spheroids. Proteolysis of DQ-collagen IV by HCT 116 and HKh-2 human colon tumor