Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC₁₈(3)))

Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC<sub>18</sub>(3)))

인용 및 참조 문헌 (1167)

Invitrogen™

Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC₁₈(3)))

Name: Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC 18 (3)))
SKU: D3911
Price: 448000 KRW

DiI은 친지질성(lipophilic) 막 염색 시약으로 세포 전체를 염색하기 위해 양뱡향으로 확산되는 특징을 가지고 있습니다. 이 염색 시약은 막에 결합하기 전까지자세히 알아보기

보기 방식 변경

카탈로그 번호	수량
D3911	25 mg
D282	100 mg

2 개 옵션

카탈로그 번호 D3911

제품 가격(KRW)

448,000

온라인 행사

Ends: 31-Mar-2026

512,000

할인액 64,000 (13%)

Each

카트에 추가하기

수량:

25 mg

제품 가격(KRW)

448,000

온라인 행사

Ends: 31-Mar-2026

512,000

할인액 64,000 (13%)

Each

카트에 추가하기

DiI은 친지질성(lipophilic) 막 염색 시약으로 세포 전체를 염색하기 위해 양뱡향으로 확산되는 특징을 가지고 있습니다. 이 염색 시약은 막에 결합하기 전까지 약한 형광을 발합니다. 이 주황빛(Orange) 적색 형광은 tetramethylrhodamine과 분광학적으로 유사하며 신경세포 및 기타 세포에 대한 장기 tracer로 사용할 수 있습니다. DiI은 용액(V-22885), 페이스트(N-22880) 또는 결정(D-3911)으로도 이용할 수 있습니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

색상Yellow

검출 방법Fluorescence

방출565 nm

여기 파장 범위549 nm

용도(장비)Fluorescence Microscope

분자량933.88

수량25 mg

배송 조건Room Temperature

제품 유형Liphophilic Tracer

SubCellular LocalizationCell Membranes, Lipids

Unit SizeEach

구성 및 보관

Store at room temperature and protect from light.

자주 묻는 질문(FAQ)

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my neurons with DiI and then fixed and permeabilized and now I have no signal. What did I do wrong?

DiI is a lipophilic dye that resides mostly in lipids in the cell, when cells are permeabilized with detergent or fixed using alcohol this strips away the lipid and the dye. If permeabilization is required CM-DiI can be used because this binds covalently to proteins in the membrane; some signal is lost upon fixation/permeabilization, but enough signal should be retained to make detection possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC₁₈(3))), 25 mg FAQs

인용 및 참조 문헌 (1167)

인용 및 참조 문헌

Abstract

Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.

Authors:Clarke JD

Journal:Methods in molecular biology (Clifton, N.J.)

PubMed ID:19030810

Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.

Authors:Morawietz H,Rueckschloss U,Niemann B,Duerrschmidt N,Galle J,Hakim K,Zerkowski HR,Sawamura T,Holtz J

Journal:Circulation

PubMed ID:10468518

Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.

Authors:Potter SM, Pine J, Fraser SE

Journal:Scanning Microsc Suppl

PubMed ID:9601539

We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/recording electrode at its base. The well is covered with a grillwork through which the ... More

Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.

Authors:Webb B, Suarez SS, Heaton MB, Walker DW

Journal:Brain Res

PubMed ID:9459553

Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More

Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.

Authors:Van Amersfoort ES, Van Strijp JA

Journal:Cytometry

PubMed ID:7875036

A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More

View all Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC₁₈(3))), 25 mg citations

Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC18(3)))

자주 묻는 질문(FAQ)

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

I labeled my neurons with DiI and then fixed and permeabilized and now I have no signal. What did I do wrong?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

인용 및 참조 문헌 (1167)

Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC₁₈(3)))