H2DCFDA (H2-DCF, DCF)
H2DCFDA (H2-DCF, DCF)
인용 및 참조 문헌 (1016)
Invitrogen™

H2DCFDA (H2-DCF, DCF)

cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (dichlorofluores cin diacetate)는 세포 내 활성산소(ROS) 표지자 등으로 사용하는 fluorescein이 화학적으로 환원된 형태로 중성구와 대식세포에서 활성산소자세히 알아보기
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카탈로그 번호수량
D399100 mg
카탈로그 번호 D399
제품 가격(KRW)
279,000
キャンペーン価格
Ends: 31-Dec-2025
309,000
할인액 30,000 (10%)
Each
카트에 추가하기
수량:
100 mg
제품 가격(KRW)
279,000
キャンペーン価格
Ends: 31-Dec-2025
309,000
할인액 30,000 (10%)
Each
카트에 추가하기
cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (dichlorofluores cin diacetate)는 세포 내 활성산소(ROS) 표지자 등으로 사용하는 fluorescein이 화학적으로 환원된 형태로 중성구와 대식세포에서 활성산소 중간체 생성을 검출합니다. 아세트산기가 intracellular esterases와 산화 반으에 의해 절단되면 비형광 H2DCFDA이 형광도가 높은 2',7'-dichlorofluorescein (DCF)로 바뀝니다. 이 보다 세포 유지력이 더 높은 표지자 버전도 구매가능합니다(C400, C2938).

ROS 표지자 사양:

• Ex/Em: ∼492–495/517–527 nm
• 제품은 공기에 민감하기 때문에 드라이 아르곤이나 질소에 보관해야 합니다.
• DMSO, DMF, 또는 에탄올에 녹여 사용할 수 있습니다.
• 이는 세포 투과성이 있습니다(세포 부하 프로토콜을 문헌에서 찾아볼 수 있습니다.)
• 형광 모니터링은 유세포 분석기나 마이크로플레이트 리더기, 형광 현미경을 사용하거나 fluorescein에 적합한 여기원(excitation sources)과 필터를 사용해 실시할 수 있습니다.

더 많은 ROS 표지자 찾기
본사는 단일 활성산호, 수퍼옥사이드, 히드록실라디칼, 다양한 퍼옥사이드, 하이드로퍼옥사이드 등 활성산호(ROX) 생성에 대한 많은 Molecular Probes™ 제품을 제공합니다. 이 제품에 대해 더 많은 정보를 원하신다면 Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook를 참조하세요.

본 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
수량100 mg
제품 유형ROS Indicator
Unit SizeEach

자주 묻는 질문(FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the recommended storage condition/shelf-life for H2DCFDA (H2-DCF, DCF) (Cat. No. D399)?

We guarantee stability of the product for a period of 3 months when stored as recommended in the freezer (-5 to -30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all H2DCFDA (H2-DCF, DCF) FAQs

인용 및 참조 문헌 (1016)

인용 및 참조 문헌
Abstract
Nitric oxide protects cultured rat hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing heat shock protein 70 expression.
Authors:Kim YM,de Vera ME,Watkins SC,Billiar TR
Journal:The Journal of biological chemistry
PubMed ID:8995451
Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.
Authors:Ying W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA
Journal:Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
PubMed ID:10698074
Authors:
Journal:
PubMed ID:11282963
Imaging of hydroperoxides in a rat glomerulus stimulated by puromycin aminonucleoside.
Authors:Aoyagi K,Akiyama K,Tomida C,Gotoh M,Hirayama A,Takemura K,Ueda A,Nagase S,Koyama A,Narita M
Journal:Kidney international. Supplement
PubMed ID:10412763
Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimodipine.
Authors:Danks AM,Hammond DN,Wainer BH,Van Buskirk RG,Isaacson RL
Journal:Brain research. Molecular brain research
PubMed ID:1334195
The immortalized septal cell line, SN56 B5 G4, generated by the fusion of mouse septal area cells and neuroblastoma cells, was used to determine if nimodipine, an antagonist of voltage sensitive calcium 'L' channels, might act in a neuroprotective fashion when intracellular calcium levels were raised by incubation in ouabain ... More
View all H2DCFDA (H2-DCF, DCF) citations