ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging
ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging
인용 및 참조 문헌 (28)
Invitrogen™

ER-Tracker™ Red (BODIPY™ TR Glibenclamide), for live-cell imaging

ER-Tracker™ Red dye는 세포 투과성의 살아있는 세포 염색제로 세포질세망(endoplasmic reticulum, ER)에 대한 선택성이 높아 제공된 프로토콜 사용 시 염색 패턴이자세히 알아보기
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카탈로그 번호수량
E34250100 μg
카탈로그 번호 E34250
제품 가격(KRW)
640,000
Online offer
Ends: 31-Dec-2025
711,000
할인액 71,000 (10%)
Each
카트에 추가하기
수량:
100 μg
제품 가격(KRW)
640,000
Online offer
Ends: 31-Dec-2025
711,000
할인액 71,000 (10%)
Each
카트에 추가하기


ER-Tracker™ Red dye
는 세포 투과성의 살아있는 세포 염색제로 세포질세망(endoplasmic reticulum, ER)에 대한 선택성이 높아 제공된 프로토콜 사용 시 염색 패턴이 포름알데히드 고정 후 부분적으로 유지됩니다. 이 염색은 녹색 형광 BODIPY™ TR dye와 glibenclamide로 이루어집니다. Glibenclamide (glyburide)는 ER에서 두드러진 ATP 민감성 K+ 채널의 sulphonylurea 수용체에 결합합니다. Glibenclamide의 약리 활성이 ER 기능에 영향을 줄 수 있습니다. 일부 특수 세포 유형에서 sulphonylurea 수용체의 가변적 발현이 비특이적인 ER 표지를 야기할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Red
검출 방법Fluorescence
여기 파장 범위587⁄615
용도(장비)Fluorescence Microscope
제품라인BODIPY, ER-Tracker
수량100 μg
배송 조건Room Temperature
라벨 유형BODIPY Dyes
제품 유형Dye
SubCellular LocalizationEndoplasmic Reticulum
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (28)

인용 및 참조 문헌
Abstract
Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands.
Authors:Peshenko IV, Olshevskaya EV, Dizhoor AM,
Journal:J Biol Chem
PubMed ID:18541533
'Guanylyl cyclase activating protein 1 (GCAP1), after substitution of Ca(2+) by Mg(2+) in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. We inactivated metal binding in individual EF-hands of GCAP1 tagged with green fluorescent protein to assess their role in GCAP1 binding to RetGC1 in co-transfected HEK293 ... More
Imaging secretory vesicles by fluorescent protein insertion in propeptide rather than mature secreted peptide.
Authors:Watkins S, Geng X, Li L, Papworth G, Robbins PD, Drain P
Journal:Traffic
PubMed ID:12047554
'We combined confocal and live-cell imaging with a novel molecular strategy aimed at revealing mechanisms underlying glucose-regulated insulin vesicle secretion. The ''Ins-C-GFP'' reporter monitors secretory peptide targeting, trafficking, and exocytosis without directly tagging the mature secreted peptide. We trapped a green fluorescent protein (GFP) reporter in equimolar quantity within the ... More
Faecal excretion of ciprofloxacin after a single oral dose and its effect on faecal bacteria in healthy volunteers.
Authors:Pecquet S, Ravoire S, Andremont A,
Journal:J Antimicrob Chemother
PubMed ID:2211433
'High concentrations of ciprofloxacin have been shown to persist in the faeces of volunteers for several days after a week of oral treatment with this drug, which was also found to have a prolonged effect on aerobic Gram-negative intestinal bacteria. To determine whether a shorter course of ciprofloxacin would have ... More
Insulin-like growth factor I enhances the expression of aromatase P450 by inhibiting autophagy.
Authors:Zhang B, Shozu M, Okada M, Ishikawa H, Kasai T, Murakami K, Nomura K, Harada N, Inoue M,
Journal:Endocrinology
PubMed ID:20668023
'Aromatase, a key enzyme of estrogen biosynthesis, is transcriptionally regulated by many growth factors. IGF-I enhances aromatase activity in a variety of cells, but the mechanism of action has not been determined. We herein report our finding of a novel mechanism of action for IGF-I. IGF-I enhanced the dexamethasone (DEX)-induced ... More
Essential requirement for sphingosine kinase 2 in a sphingolipid apoptosis pathway activated by FTY720 analogues.
Authors:Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H
Journal:J Biol Chem
PubMed ID:17400555
'The clinical immunosuppressant FTY720 is a sphingosine analogue that, once phosphorylated by sphingosine kinase 2 (Sphk2), is an agonist of multiple receptor subtypes for sphingosine 1-phosphate. Short exposures to FTY720 afford long term protection in lymphoproliferative and autoimmune disease models, presumably by inducing apoptosis in subsets of cells essential for ... More
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