ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging
ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging
인용 및 참조 문헌 (28)
Invitrogen™

ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

ER-Tracker™ Green이 제품은 세포 투과성이 높아 live cell의 염색이 가능하며, 소포체(ER)에 대하여 선택적으로 결합합니다. 제공된 프로토콜 사용 시 염색 패턴이자세히 알아보기
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카탈로그 번호수량
E34251100 μg
카탈로그 번호 E34251
제품 가격(KRW)
639,000
Online offer
Ends: 31-Dec-2025
710,000
할인액 71,000 (10%)
Each
카트에 추가하기
수량:
100 μg
제품 가격(KRW)
639,000
Online offer
Ends: 31-Dec-2025
710,000
할인액 71,000 (10%)
Each
카트에 추가하기


ER-Tracker™ Green
이 제품은 세포 투과성이 높아 live cell의 염색이 가능하며, 소포체(ER)에 대하여 선택적으로 결합합니다. 제공된 프로토콜 사용 시 염색 패턴이 포름알데히드 고정 후 부분적으로 유지됩니다. 이 제품은 녹색 형광 BODIPY™ FL dye와 glibenclamide로 이루어졌습니다. Glibenclamide (glyburide)는 ER에서 두드러진 ATP 민감성 K+ 채널의 sulphonylurea 수용체에 결합합니다. Glibenclamide의 약리 활성이 ER 기능에 영향을 줄 수 있습니다. 일부 특수한 세포에서 sulphonylurea 수용체의 발현이 ER 표지를 억제할 수 있습니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Green
검출 방법Fluorescence
용도(장비)Fluorescence Microscope
제품라인BODIPY, ER-Tracker
수량100 μg
배송 조건Room Temperature
라벨 유형BODIPY Dyes
제품 유형Dye
SubCellular LocalizationEndoplasmic Reticulum
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (28)

인용 및 참조 문헌
Abstract
Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.
Authors:Guiot Y, Stevens M, Marhfour I, Stiernet P, Mikhailov M, Ashcroft SJ, Rahier J, Henquin JC, Sempoux C,
Journal:Diabetologia
PubMed ID:17593344
'AIMS/HYPOTHESIS: Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive K channels in beta cells. Morphological methods (immunohistochemistry and sulfonylurea binding) were used to establish the cellular and subcellular location of SUR1 in human and rodent islets. RESULTS: In the human, mouse and rat pancreas, all endocrine cells of ... More
Live intracellular super-resolution imaging using site-specific stains.
Authors:Carlini L, Manley S,
Journal:
PubMed ID:24079385
'Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several ... More
Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis.
Authors:Mirnikjoo B, Balasubramanian K, Schroit AJ,
Journal:J Biol Chem
PubMed ID:19561081
One of the hallmarks of apoptosis is the redistribution of phosphatidylserine (PS) from the inner-to-outer plasma membrane (PM) leaflet, where it functions as a ligand for phagocyte recognition and the suppression of inflammatory responses. The mechanism by which apoptotic cells externalize PS has been assumed to involve  ... More
Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.
Authors:Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,
Journal:J Biol Chem
PubMed ID:18281291
Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More
Translocation of a phycoerythrin alpha subunit across five biological membranes.
Authors:Gould SB, Fan E, Hempel F, Maier UG, Klösgen RB,
Journal:J Biol Chem
PubMed ID:17702756
Cryptophytes, unicellular algae, evolved by secondary endosymbiosis and contain plastids surrounded by four membranes. In contrast to cyanobacteria and red algae, their phycobiliproteins do not assemble into phycobilisomes and are located within the thylakoid lumen instead of the stroma. We identified two gene families encoding phycoerythrin alpha and light-harvesting complex ... More
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