The ELF™ 97 Endogenous Phosphatase Kit can be used to detect phosphatase activity with the ELF™ 97 phosphatase substrate that자세히 알아보기
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카탈로그 번호
수량
E6601
1 kit
카탈로그 번호 E6601
제품 가격(KRW)
595,000
キャンペーン価格
Ends: 31-Mar-2026
699,000
할인액 104,000 (15%)
Each
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수량:
1 kit
제품 가격(KRW)
595,000
キャンペーン価格
Ends: 31-Mar-2026
699,000
할인액 104,000 (15%)
Each
카트에 추가하기
The ELF™ 97 Endogenous Phosphatase Kit can be used to detect phosphatase activity with the ELF™ 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형ELF 97
형태Liquid
수량1 kit
배송 조건Wet Ice
기질ELF™ 97 phosphate
기질 특성Chemical Substrate
기질 유형Phosphatase Substrate
타겟 효소Phosphatases
색상Yellow-Green
Emission345/530
용도(애플리케이션)Phosphatase Assay
용도 (장비)Fluorescence Microscope
제품라인ELF
제품 유형Phosphatase Assay
Unit SizeEach
구성 및 보관
Store in refrigerator 2°C to 8°C and protect from light.
자주 묻는 질문(FAQ)
Can the ELF 97 reagent be applied to live cells?
ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).
Primitive adult hematopoietic stem cells can function as osteoblast precursors.
Authors:Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR
Journal:Proc Natl Acad Sci U S A
PubMed ID:14673088
'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here ... More
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
Journal:Stem Cells
PubMed ID:17284651
'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More
Osteoclast responses to lipopolysaccharide, parathyroid hormone and bisphosphonates in neonatal murine calvaria analyzed by laser scanning confocal microscopy.
'Because the development and activity of osteoclasts in bone remodeling is critically dependent on cell-cell and cell-matrix interactions, we used laser confocal microscopy to study the response of osteoclasts to lipopolysaccharide (LPS; 10 microg/ml), parathyroid hormone (PTH; 10(-8) M), and bisphosphonates (BPs; 1-25 microM clodronate or 0.1-2.5 microM risedronate) in ... More
Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols.
Authors:Filgueira L
Journal:J Histochem Cytochem
PubMed ID:14966208
Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ... More