EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays
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인용 및 참조 문헌 (19)
Invitrogen™

EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

The EnzChek™ Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid,자세히 알아보기
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카탈로그 번호수량
E66381 Kit
카탈로그 번호 E6638
제품 가격(KRW)
677,000
Online offer
Ends: 31-Mar-2026
773,000
할인액 96,000 (12%)
Each
카트에 추가하기
수량:
1 Kit
제품 가격(KRW)
677,000
Online offer
Ends: 31-Mar-2026
773,000
할인액 96,000 (12%)
Each
카트에 추가하기
The EnzChek™ Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.

See our complete line of Fluorescence Microplate assays.

  • Generalized mechanism allows for measurement of a variety of proteases
  • Can be used to continuously measure protease kinetics
  • Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings

    This EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY™ FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY™ FL dye has excitation and emission spectra similar to those of fluorescein.

    • For Research Use Only. Not for use in diagnostic procedures.
    사양
    검출 방법Fluorescence Intensity
    수량1 Kit
    연구 카테고리Differentiation
    배송 조건Room Temperature
    기질 특성Protein-Based Substrate
    기질 유형Protease Substrate
    타겟 효소Proteases
    용도(애플리케이션)Protease Assay
    용도 (장비)Fluorescence Microplate Reader
    제품라인EnzChek
    제품 유형EnzChek Assay Kit
    Unit SizeEach
    구성 및 보관
    Store in freezer -5°C to -30°C and protect from light.

    자주 묻는 질문(FAQ)

    What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

    The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

    What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

    As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    인용 및 참조 문헌 (19)

    인용 및 참조 문헌
    Abstract
    Authors:
    Journal:
    PubMed ID:10748021
    Effective antiprotease-antibiotic treatment of experimental anthrax.
    Authors:Popov SG, Popova TG, Hopkins S, Weinstein RS, MacAfee R, Fryxell KJ, Chandhoke V, Bailey C, Alibek K
    Journal:BMC Infect Dis
    PubMed ID:15819985
    'BACKGROUND: Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of ... More
    Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices.
    Authors:Peterson DS, Rohr T, Svec F, Fréchet JM
    Journal:Anal Chem
    PubMed ID:12199578
    'Enzymatic microreactors have been prepared in capillaries and on microfluidic chips by immobilizing trypsin on porous polymer monoliths consisting of 2-vinyl-4,4-dimethylazlactone, ethylene dimethacrylate, and acrylamide or 2-hydroxyethyl methacrylate. The azlactone functionalities react readily with amine and thiol groups of the enzyme to form stable covalent bonds. The optimized porous properties ... More
    Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.
    Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP
    Journal:Anal Biochem
    PubMed ID:9299009
    'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More
    Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
    Authors:Nolkrantz K, Farre C, Hurtig KJ, Rylander P, Orwar O
    Journal:Anal Chem
    PubMed ID:12199607
    'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased ... More
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