DH10B Competent Cells
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DH10B Competent Cells
Thermo Scientific™

DH10B Competent Cells

Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning자세히 알아보기
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카탈로그 번호수량
EC011310 x 100 μL
카탈로그 번호 EC0113
제품 가격(KRW)
231,000
キャンペーン価格
Ends: 31-Mar-2026
263,000
할인액 32,000 (12%)
Each
카트에 추가하기
수량:
10 x 100 μL
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
231,000
キャンペーン価格
Ends: 31-Mar-2026
263,000
할인액 32,000 (12%)
Each
카트에 추가하기
Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Allows for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning both of prokaryotic and eukaryotic origin
• Cloning of large plasmids and BACs
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site directed mutagenesis

Genotype
F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주DH10B™
블루/화이트 스크리닝Yes
세포 유형Chemically Competent
메틸화 DNA 클로닝Yes
에프에피솜 포함Lacks F' Episome
효율High Efficiency (>1x10^9 cfu/μg pUC19 DNA)
용도(애플리케이션)Cloning
고처리량 호환성Not High-throughput Compatible
라이브러리cDNA
제품라인Thermo Scientific
제품 유형Competent Cell
ccdB 벡터 증식Not for ccdB vector propagation
수량10 x 100 μL
배송 조건Dry Ice
형식Tube
종E. coli
Unit SizeEach
구성 및 보관
• 10 x DH10B Competent Cells (100 μL)
• pUC19 DNA (50 μL) (10 pg/μL)
• S.O.C. Medium (5 mL)

Store at -80°C.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

I'm getting overgrowth of colonies. Why?

Ensure that you are using the correct antibiotic at the appropriate concentration. Additionally, make sure the antibiotic is not expired. If colonies exhibit unexpected morphologies, contamination could be a factor. Check your S.O.C. medium and LB growth medium.

I'm only getting white colonies, but none of the clones have an insert. What can I do?

Here are a few suggestions:

- Small fragments/linkers are cloning in to your vector instead of your insert; to correct this, gel-purify the insert before ligation
- Ensure that the correct concentrations of X-gal and/or IPTG (if vector contains the lacIq marker) are used
- If spreading X-gal and/or IPTG on your plate, allow sufficient time for the reagents to diffuse into the plate
- Incubate your plate for a longer period to ensure full color development

I'm getting no colonies at all on my plates. Can you help?

We recommend trying the following:
- Carry out the puc19 transformation control; this gives you information about the performance of the cells.
- Check plates for expiration and correct media used (LB/agar).
- Confirm that the correct antibiotic and concentration was used.

View all DH10B Competent Cells FAQs