β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Invitrogen™

β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

β-hydroxybutyrate (β-HB), C4H8O3, accounts for about 75% of the total ketone body. Patients with diabetic ketoacidosis have increased production of NADH, which promotes the conversion of acetoacetic acid into β-HB. Therefore, the level of β-hydroxybutyrate can be used as an index to evaluate glycosuria.
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카탈로그 번호수량
EEA04696 Tests
카탈로그 번호 EEA046
제품 가격(KRW)
-
수량:
96 Tests

With this kit, β-hydroxybutyrate dehydrogenase catalyzes the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfers electrons to WST-8 to produce a yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm. This kit is useful for researchers studying ketone body metabolism and diabetes.

Key features

  • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
  • Broad range of kits across key analytes and targets within the most important metabolic pathways
  • Consistent—strict quality control criteria to ensure reproducible and reliable results
For Research Use Only.
사양
어세이 범위0.01 to 2.00 mmol/L
어세이 민감성0.01 mmol/L
용도(장비)Microtiter Plate Reader
인큐베이션 시간40 min.
분석 간 CV4.0%
분석 내 CV2.0%
제품 유형Kit
수량96 Tests
샘플 부피10 μL
배송 조건Dry Ice, 2°C to 8°C
타겟 키트 이름 지정β-Hydroxybutyrate (Ketone Body)
총 어세이 시간60 min.
검출 방법Colorimetric
이뮤노어세이 키트 형식Colorimetric Detection Kit
샘플 종류Serum, Plasma, Urine, Animal Tissue
타겟 종Universal
Unit SizeEach
구성 및 보관
Store at 2°C to 8°C.