Serum Creatinine Colorimetric Detection Kit

The Serum Creatinine Colorimetric Detection Kit is designed to detect and quantify the level of creatinine in serum and plasma samples. The assay was validated for human, mouse, rabbit, rat, and sheep serum, EDTA plasma, and heparin plasma samples. This complete, ready-to-use kit includes clear two 96-well plates, creatinine standard, assay diluent, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 490 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum and plasma (EDTA and heparin)
• Sensitivity: 0.081 μg/dL
• Standard curve range: 0.5–4 mg/dL
• Reactivity: species independent

Assay principle
The Serum Creatinine Colorimetric Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls, and samples. The color-generating reaction is initiated with the Creatinine Reagent, which is pipetted into each well.

The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490 nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards.