E. coli DNA Ligase

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.

Features

• Activity in wide temperature range (4°C to 37°C)
• High specificity to cohesive DNA ends

Applications

• Nick ligation in double-stranded DNA
• cDNA synthesis
• Cloning