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Thermo Scientific™
Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
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보기 방식 변경
| 카탈로그 번호 | 형식 |
|---|---|
| EP0072 | With BSA |
| EP0282 | Without BSA |
카탈로그 번호 EP0072
제품 가격(KRW)
228,000
Each
형식:
With BSA
제품 가격(KRW)
228,000
Each
Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.
Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail.
Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific priming
Features
- Thermostable with half-life more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- DNA labeling
- High throughput PCR
Notes
- The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
- The 10X Taq Buffer without Detergent is recommended for microarray experiments.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식With BSA
최적 반응 온도72°C
중합효소Taq DNA Polymerase
제품 유형DNA Polymerase
수량500 units
연구 카테고리PCR
배송 조건Dry Ice
농도5 U/μL
용도(애플리케이션)Standard PCR
Unit SizeEach
구성 및 보관
• Taq DNA Polymerase, native (5 U/μL), 100 μL
• 10X Taq Buffer with KCl, 2 x 1.25 mL
• 10X Taq Buffer with (NH4)2SO4, 2 x 1.25 mL
• 25 mM MgCl2, 2 x 1.25 mL
• BSA (20 mg/mL), 0.25 mL
Store at -20°C.
• 10X Taq Buffer with KCl, 2 x 1.25 mL
• 10X Taq Buffer with (NH4)2SO4, 2 x 1.25 mL
• 25 mM MgCl2, 2 x 1.25 mL
• BSA (20 mg/mL), 0.25 mL
Store at -20°C.