Fluo-4 Direct™ Calcium Assay Kit
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인용 및 참조 문헌 (8)
Invitrogen™

Fluo-4 Direct™ Calcium Assay Kit

Fluo-4 Direct™ Calcium Assay Kit는 다음과 같은 균일한 형광 칼슘 분석을 제공하도록 구성되었습니다.1. 세포로의 손쉬운 loading2. 넓은 분석 허용범위3. 특정자세히 알아보기
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카탈로그 번호수량
F104731 x 1 L
F1047110 x 10 mL
F104721 x 100 mL
카탈로그 번호 F10473
제품 가격(KRW)
6,525,000
온라인 행사
Ends: 31-Dec-2025
7,676,000
할인액 1,151,000 (15%)
Each
카트에 추가하기
수량:
1 x 1 L
제품 가격(KRW)
6,525,000
온라인 행사
Ends: 31-Dec-2025
7,676,000
할인액 1,151,000 (15%)
Each
카트에 추가하기
Fluo-4 Direct™ Calcium Assay Kit는 다음과 같은 균일한 형광 칼슘 분석을 제공하도록 구성되었습니다.
1. 세포로의 손쉬운 loading
2. 넓은 분석 허용범위
3. 특정 세포 형광에 거의 영향을 미치지 않고 완전한 배지에서 칼슘 표지자로 야기되는 background 형광 차단

개선된 formulation은 혈청 함유 배지에서도 첨가만 하면 되는 형식으로 분석을 간편하게 합니다. Fluo-4 Direct™는 Fluo-4 칼슘 검출 시약군의 세번째로 개발된 제품입니다. Fluo-4 AM 및 Fluo-4 NW는 모두 검출 분석 전 배지를 제거해야 하나 Fluo-4 NW는 편리함을 더해 PowerLoad™ 시약을 세포 loading에 첨가하기만 하면 됩니다. Fluo-4 Direct™ Calcium Assay Kit는 손쉬운 세포 loading을 위해 PowerLoad™로 만들어졌다는 면에서 Fluo-4 NW와 비슷하지만 완전 배양 배지(complete media)에 사용할 수 있고 분석에서 발생하는 특정 세포 형광을 희생시키지 않고 효율적으로 background 형광을 차단할 수 있다는 특징을 갖습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형Fluorescent Dye-Based
형태Powder
수정Chemical
수량1 x 1 L
배송 조건Room Temperature
세포하위구조 국지화Nucleus, Organelles, Cytoplasm
색상Green
EmissionVisible
용도(애플리케이션)Cell Viability and Proliferation
용도 (장비)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
제품라인Molecular Probes
제품 유형Calcium Assay Kit
Unit SizeEach
구성 및 보관
  • 1 L Fluo-4 Direct™ Calcium Assay Reagent (Component A)
  • 1.54 g Probenecid (Component B)
  • Store at ≤-20°C. Dessicate and protect from light.

자주 묻는 질문(FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (8)

인용 및 참조 문헌
Abstract
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,
Journal:J R Soc Interface
PubMed ID:21490003
'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More
20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.
Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,
Journal:J Biol Chem
PubMed ID:22389490
'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More
Selective Impairment of P2Y Signaling by Prostaglandin E2 in Macrophages: Implications for Ca2+-Dependent Responses.
Authors:Través PG, Pimentel-Santillana M, Carrasquero LM, Pérez-Sen R, Delicado EG, Luque A, Izquierdo M, Martín-Sanz P, Miras-Portugal MT, Boscá L,
Journal:J Immunol
PubMed ID:23479225
Extracellular nucleotides have been recognized as important modulators of inflammation via their action on specific pyrimidine receptors (P2). This regulation coexists with the temporal framework of proinflammatory and proresolution mediators released by the cells involved in the inflammatory response, including macrophages. Under proinflammatory conditions, the expression of cyclooxygenase-2 leads to ... More
Surfactant protein A integrates activation signal strength to differentially modulate T cell proliferation.
Authors:Mukherjee S, Giamberardino C, Thomas J, Evans K, Goto H, Ledford JG, Hsia B, Pastva AM, Wright JR,
Journal:J Immunol
PubMed ID:22219327
Pulmonary surfactant lipoproteins lower the surface tension at the alveolar-airway interface of the lung and participate in host defense. Previous studies reported that surfactant protein A (SP-A) inhibits lymphocyte proliferation. We hypothesized that SP-A-mediated modulation of T cell activation depends upon the strength, duration, and type of lymphocyte activating signals. ... More
HIV-1 Tat protein inhibits neurosecretion by binding to phosphatidylinositol 4,5-bisphosphate.
Authors:Tryoen-Tóth P, Chasserot-Golaz S, Tu A, Gherib P, Bader MF, Beaumelle B, Vitale N,
Journal:J Cell Sci
PubMed ID:23178941
HIV-1 transcriptional activator (Tat) enables viral transcription and is also actively released by infected cells. Extracellular Tat can enter uninfected cells and affect some cellular functions. Here, we examine the effects of Tat protein on the secretory activity of neuroendocrine cells. When added to the culture medium of chromaffin and ... More
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