Search
Thermo Scientific™
Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Have Questions?
보기 방식 변경
| 카탈로그 번호 | 색상 | 반응 수 |
|---|---|---|
| F122L | Colorless | 1000 Reactions |
| F122S | Colorless | 200 Reactions |
| F124L | Green | 1000 Reactions |
| F124S | Green | 200 Reactions |
카탈로그 번호 F122L
제품 가격(KRW)
837,000
온라인 행사
Ends: 31-Dec-2025
984,000할인액 147,000 (15%)
Each
색상:
Colorless
반응 수:
1000 Reactions
제품 가격(KRW)
837,000
온라인 행사
Ends: 31-Dec-2025
984,000할인액 147,000 (15%)
Each
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10–15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase (Cat. No. EP0072).
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Features
- No reactivation step with hot start
- Amplification four times faster than hot start Taq
- Minimal reaction optimization due to high inhibitor tolerance
- Abundant products due to high efficiency
- Amplifies significantly longer DNA fragments than hot start Taq
- Direct loading on gel with Green Buffer
Applications
- Hot Start PCR
- Standard PCR
- Non-high-fidelity PCR
- Fast PCR
- High throughput PCR
- Genotyping
Notes
- The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Colorless
Fidelity (Taq 대비)2X
핫 스타트Built-In Hot Start
반응 수1000 Reactions
오버행Blunt
중합효소Phire Hot Start II DNA Polymerase
제품 유형DNA Polymerase
순도 또는 품질 등급DHPLC
수량1,000 reactions
반응 형식Separate Components
배송 조건Wet or Dry Ice
시작 물질DNA
용도(애플리케이션)Cloning, Hot-start PCR
GC-Rich PCR PerformanceMedium
반응 속도Fast
Unit SizeEach
구성 및 보관
• Phire Hot Start II DNA Polymerase, 1 x 1 mL
• 5X Phire Reaction Buffer, 7 x 1.5 mL
• DMSO, 1 x 500 μL
Store at -20°C.
• 5X Phire Reaction Buffer, 7 x 1.5 mL
• DMSO, 1 x 500 μL
Store at -20°C.