CellLight™ FUCCI Cell Cycle Indicator

CellLight™ FUCCI Cell Cycle Indicator is a fixable live-cell imaging reagent that works as a cell cycle dye alternative, labeling nuclei red in G1, green in S/G2/M, and yellow at G1/S. The reagent combines GFP-geminin and mKate-Cdt1 in a single BacMam vector, co-tranducing both reporters while supporting imaging with standard GFP and Texas Red filter sets. It is added directly to cells in complete medium, typically read after 16 to 24 hours, and can be carried into fixation and permeabilization workflows for downstream staining or endpoint phenotyping.

A single BacMam particle co-tranduces GFP-geminin and mKate-Cdt1 from a non-replicating baculovirus-based construct supporting equal delivery of both fluorescent proteins with a single transduction.

CellLight™ FUCCI supports imaging with standard GFP and Texas Red filter sets and excels for timelapse imaging for longitudinal or perturbation studies.

As a transduction reagent the BacMam vector has an excellent safety profile and offers a transient approach in mammalian cells because the baculovirus does not replicate in mammalian hosts.

Features

• Added directly to cells in complete culture medium, with fluorescence typically assessed after 16 to 24 hours.
• Compatible with standard fixation and permeabilization workflows, supporting endpoint antibody staining or other fluorescent markers after live-cell imaging.
• Fits microscopy and high-content imaging workflows used to assess proliferation, cell-cycle perturbation, or population shifts after drug, siRNA, or other experimental treatment.

Transient episomal expression supports short-term studies and with optional continuous culture use when sustained labeling is needed. Optimizing from a starting range of 10 to 100 particles per cell for standard mammalian cultured cells with noted lower performance in hematopoietic lineages.

The CellLight™ FUCCI workflow is compatible with routine cell culture with samples moving into downstream staining with antibodies, landmark dyes, or other endpoint markers after live-cell imaging.