Phire Plant Direct PCR Kit (without sampling tools)
Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific™

Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
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카탈로그 번호수량
F130WH500 reactions
카탈로그 번호 F130WH
제품 가격(KRW)
412,000
キャンペーン価格
Ends: 31-Mar-2026
470,000
할인액 58,000 (12%)
Each
카트에 추가하기
수량:
500 reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
412,000
キャンペーン価格
Ends: 31-Mar-2026
470,000
할인액 58,000 (12%)
Each
카트에 추가하기
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria.

The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions. The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Very little sample material required
  • Two simple protocols for various applications
  • Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb)

Applications

  • Genotyping
  • Transgene detection

Notes

  • The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
사양
핫 스타트Built-In Hot Start
반응 수500 Reactions
오버행Blunt
중합효소Phire Hot Start II DNA Polymerase
제품 유형Direct PCR Kit
수량500 reactions
반응 형식Separate Components
샘플 종류Plant
배송 조건Wet or Dry Ice
시작 물질DNA
GC-Rich PCR PerformanceMedium
PCR 방법Direct PCR
반응 속도Fast
Unit SizeEach
구성 및 보관
• Phire Hot Start II DNA Polymerase, 200 μL
• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL
• Universal Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL

Store at -20°C.

자주 묻는 질문(FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

Do the Direct PCR Master Mixes and Kits come with tissue punchers?

No, tissue punchers are not provided. You can purchase 0.3 mm Tissue Punchers (Cat. No. F-200S), or cut the sample with a scalpel. Some scientists use 100 µL pipette tips to take a sample.

View all Phire Plant Direct PCR Kit (without sampling tools) FAQs