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Fluo-4, AM, cell permeant
Fluo-4, AM, cell permeant
인용 및 참조 문헌 (323)
Invitrogen™

Fluo-4, AM, cell permeant

표지된 칼슘 표지자는 Ca2+ 결합 시 형광이 증강되는 분자입니다. Fluo-3는 caged chelators, second messenger, neurotransmitter의 광활성이 관여한 유세포측정 실험과 세포기반자세히 알아보기
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카탈로그 번호수량
F1420110 x 50 μg
카탈로그 번호 F14201
제품 가격(KRW)
503,000
Exklusiv online
Ends: 31-Mar-2026
558,000
할인액 55,000 (10%)
Each
카트에 추가하기
수량:
10 x 50 μg
제품 가격(KRW)
503,000
Exklusiv online
Ends: 31-Mar-2026
558,000
할인액 55,000 (10%)
Each
카트에 추가하기
표지된 칼슘 표지자는 Ca2+ 결합 시 형광이 증강되는 분자입니다. Fluo-3는 caged chelators, second messenger, neurotransmitter의 광활성이 관여한 유세포측정 실험과 세포기반 약물 스크리닝에서 Ca2+ 신호 공간역학 영상 촬영에 사용되고 있습니다. Fluo-4는 fluorine 대신 chlorine substituent 2개가 있는 fluo-3 유사체로 488 nm에서 형광 여기가 증가하기 때문에 높은 형광 신호를 제공합니다. 용해된 표지자를 세포 배양 접시에 직접 첨가하여 이런 칼슘 표지자의 AM ester forms을 세포에 부하할 수 있습니다. 이런 표지자는 형광현미경 및 공초점현미경 시험, 유세포측정, 마이크로플레이트 스크리닝 어플리케이션에 유용합니다.

칼슘 표지자(AM Ester) 사양:

• 라벨 (Ca2+–결합형 Ex/Em): Fluo-4 (494/506 nm)
• Ca2+ 결합 시 형광 강도 증강: >100 fold
• 버퍼에서 Ca2+의 Kd: ∼335 nM
• 파장 변화가 거의 없이 Ca2+ 결합 시 형광 증강

TPEN을 사용해 중금속 양이온 제어
또한, 이와 같은 BAPTA 기반 표지자는 다양한 중금속 양이온(예: Mn2+, Zn2+, Pb2+)에 Ca2+ 보다 매우 높은 친화성으로 결합함. 중금속 선택적 킬레이터 TPEN를 사용해 이런 이온의 유무로 유발되는 칼슘 측정 변화를 제어할 수 있습니다.

그 밖의 형광 칼슘 표지자
본사는 다양한 실험에 사용할 수 있는 폭넓은 Molecular Probes® 칼슘 표지자를 제공합니다. 자세한 정보는 Molecular Probes® 핸드북에서 가시광선 여기 형광 Ca2+ 표지자—Section 19.3을 참조하십시오.

UV 여기 Ca2+ 표지자, 단백질 기반 Ca2+ 표지자, Ca2+ conjugate 표지자, 기타 금속 이온(i.e., Mg2+, Zn2+)의 형광 기반 표지자는 Molecular Probes® 핸드북에서Ca2+, Mg2+, Zn2+ 및 기타 금속 이온 표지자—Chapter 19를 참조하십시오.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형Fluorescent Dye-Based
여기/방출494/506 nm
수량10 x 50 μg
배송 조건Room Temperature
용도(애플리케이션)Calcium Indicator, Cell Proliferation, Cellular Imaging
용도 (장비)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
제품 유형Dye
Unit SizeEach
구성 및 보관
Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

Can I fix my cells after loading with Fluo-4 AM dye for the detection of calcium?

No. Since Fluo-4 AM isn't covalently bound to any cellular components and fixation compromises the membrane, the dye would not be retained by the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (323)

인용 및 참조 문헌
Abstract
Functional implications of calcium permeability of the channel formed by pannexin 1.
Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N
Journal:The Journal of cell biology
PubMed ID:16908669
Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More
Impaired insulin secretion and glucose intolerance in synaptotagmin-7 null mutant mice.
Authors:Gustavsson N,Lao Y,Maximov A,Chuang JC,Kostromina E,Repa JJ,Li C,Radda GK,Südhof TC,Han W
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:18308938
Vertebrates express at least 15 different synaptotagmins with the same domain structure but diverse localizations and tissue distributions. Synaptotagmin-1,-2, and -9 act as calcium sensors for the fast phrase of neurotransmitter release, and synaptotagmin-12 acts as a calcium-independent modulator of release. The exact functions of the remaining 11 synaptotagmins, however, ... More
High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening.
Authors:Young SM, Curry MS, Ransom JT, Ballesteros JA, Prossnitz ER, Sklar LA, Edwards BS
Journal:J Biomol Screen
PubMed ID:15006133
HyperCyt, an automated sample handling system for flow cytometry that uses air bubbles to separate samples sequentially introduced from multiwell plates by an autosampler. In a previously documented HyperCyt configuration, air bubble separated compounds in one sample line and a continuous stream of cells in another are mixed in-line for ... More
Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo.
Authors:Bronk P, Wenniger JJ, Dawson-Scully K, Guo X, Hong S, Atwood HL, Zinsmaier KE
Journal:Neuron
PubMed ID:11395008
'Previous in vitro studies of cysteine-string protein (CSP) imply a potential role for the clathrin-uncoating ATPase Hsc70 in exocytosis. We show that hypomorphic mutations in Drosophila Hsc70-4 (Hsc4) impair nerve-evoked neurotransmitter release, but not synaptic vesicle recycling in vivo. The loss of release can be restored by increasing external or ... More
Multiplex GPCR assay in reverse transfection cell microarrays.
Authors:Mishina YM, Wilson CJ, Bruett L, Smith JJ, Stoop-Myer C, Jong S, Amaral LP, Pedersen R, Lyman SK, Myer VE, Kreider BL, Thompson CM
Journal:J Biomol Screen
PubMed ID:15140381
'G protein-coupled receptors (GPCRs) are a superfamily of proteins that include some of the most important drug targets in the pharmaceutical industry. Despite the success of this group of drugs, there remains a need to identify GPCR-targeted drugs with greater selectivity, to develop screening assays for validated targets, and to ... More
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