Fluo-5N, Pentapotassium Salt, cell impermeant
Fluo-5N, Pentapotassium Salt, cell impermeant
인용 및 참조 문헌 (7)
Invitrogen™

Fluo-5N, Pentapotassium Salt, cell impermeant

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs자세히 알아보기
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카탈로그 번호수량
F14203
F-14203으로도 사용됨
500 μg
카탈로그 번호 F14203
F-14203으로도 사용됨
제품 가격(KRW)
574,000
Each
카트에 추가하기
수량:
500 μg
제품 가격(KRW)
574,000
Each
카트에 추가하기
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca2+-binding affinity, making them suitable for detecting intracellular calcium levels in the 1 μM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or our Influx™ pinocytotic cell-loading reagent. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Calcium Indicator (Cell-Impermeant Salts) Specifications:

  • Label (Ex/Em of Ca2+–bound form): Fluo-5N (494/516 nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in buffer: ∼90 μM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength

    Using TPEN to Control Heavy Metal Cations

    In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

    More Choices for Fluorescent Calcium Indicators

    We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

    For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    • For Research Use Only. Not for use in diagnostic procedures.
    사양
    검출 방법Fluorescence
    염료 유형Fluorescent Dye-Based
    수량500 μg
    배송 조건Room Temperature
    용도 (장비)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
    제품 유형Calcium Indicator
    Unit SizeEach
    구성 및 보관
    Store in freezer -5°C to -30°C and protect from light.

    인용 및 참조 문헌 (7)

    인용 및 참조 문헌
    Abstract
    Rational design of protein-based MRI contrast agents.
    Authors:Yang JJ, Yang J, Wei L, Zurkiya O, Yang W, Li S, Zou J, Zhou Y, Maniccia AL, Mao H, Zhao F, Malchow R, Zhao S, Johnson J, Hu X, Krogstad E, Liu ZR,
    Journal:J Am Chem Soc
    PubMed ID:18576649
    We describe the rational design of a novel class of magnetic resonance imaging (MRI) contrast agents with engineered proteins (CAi.CD2, i = 1, 2,..., 9) chelated with gadolinium. The design of protein-based contrast agents involves creating high-coordination Gd(3+) binding sites in a stable host protein using amino acid residues and ... More
    Concurrent maturation of inner hair cell synaptic Ca2+ influx and auditory nerve spontaneous activity around hearing onset in mice.
    Authors:Wong AB, Jing Z, Rutherford MA, Frank T, Strenzke N, Moser T,
    Journal:
    PubMed ID:23804089
    Hearing over a wide range of sound intensities is thought to require complementary coding by functionally diverse spiral ganglion neurons (SGNs), each changing activity only over a subrange. The foundations of SGN diversity are not well understood but likely include differences among their inputs: the presynaptic active zones (AZs) of ... More
    Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.
    Authors:Hollingworth S, Gee KR, Baylor SM,
    Journal:Biophys J
    PubMed ID:19804716
    The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly ... More
    Tuning of synapse number, structure and function in the cochlea.
    Authors:Meyer AC, Frank T, Khimich D, Hoch G, Riedel D, Chapochnikov NM, Yarin YM, Harke B, Hell SW, Egner A, Moser T,
    Journal:Nat Neurosci
    PubMed ID:19270686
    Cochlear inner hair cells (IHCs) transmit acoustic information to spiral ganglion neurons through ribbon synapses. Here we have used morphological and physiological techniques to ask whether synaptic mechanisms differ along the tonotopic axis and within IHCs in the mouse cochlea. We show that the number of ribbon synapses per IHC ... More
    Confocal imaging of CICR events from isolated and immobilized SR vesicles.
    Authors:Shannon TR, Bers DM, Blatter LA, Niggli E
    Journal:Cell Calcium
    PubMed ID:16122794
    The Ca2+ concentration inside the sarcoplasmic reticulum ([Ca2+]SR) is a difficult parameter to measure in ventricular cardiac myocytes. Interference from Ca2+-sensitive dye loading into cellular compartments other than the SR interferes with free Ca2+ measurement. In addition, the composition of the cytosol surrounding the SR in intact cells cannot be ... More
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