FluoCells prepared microscope slides contain multi-labeled cell preparations for observation by fluorescence microscopy. FluoCells Prepared Slide #2 contains bovine pulmonary artery endothelial cells (BPAEC). Within each cell, F-actin is labelled with Texas Red-X phalloidin, microtubules are labelled with anti–bovine α-tubulin mouse monoclonal 236-10501 in conjunction with AlexaFluor 488 goat anti–mouse IgG antibody, and the nucleus is labelled with DAPI.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Immunofluorescence
수량1 Each
배송 조건Room Temperature
제품라인BODIPY FluoCells Texas Red
유형Prepared Microscope Slide
Unit SizeEach
구성 및 보관
Store at room temperature and protect from light.
자주 묻는 질문(FAQ)
Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?
Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.
DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?
DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.
Practical confocal microscopy and the evaluation of system performance.
Authors:Zucker RM, Price OT
Journal:Methods
PubMed ID:10491274
'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More