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Thermo Scientific™
Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
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보기 방식 변경
| 카탈로그 번호 | 반응 수 |
|---|---|
| F160S | 250 Reactions |
| F160L | 1250 Reactions |
카탈로그 번호 F160S
제품 가격(KRW)
225,000
Each
반응 수:
250 Reactions
제품 가격(KRW)
225,000
Each
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.
Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.
Features
- Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows several PCR reactions from one sample
- Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
사양
핫 스타트Built-In Hot Start
라벨 또는 염료 포함Yes
반응 수250 Reactions
오버행Blunt
중합효소Phire Hot Start II DNA Polymerase
제품 유형Direct PCR Master Mix
수량250 reactions
반응 형식SuperMix or Master Mix
샘플 종류Plant
배송 조건Wet or Dry Ice
시작 물질DNA
농도2X
용도(애플리케이션)Genotyping, Hot-start PCR
GC-Rich PCR PerformanceMedium
PCR 방법Direct PCR
반응 속도Fast
Unit SizeEach
구성 및 보관
• 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 1 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
Store at -20°C.
• Dilution Buffer, 1 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
Store at -20°C.