Phire Tissue Direct PCR Master Mix
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Phire Tissue Direct PCR Master Mix
Thermo Scientific™

Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
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카탈로그 번호반응 수
F170S250 Reactions
F170L1250 Reactions
카탈로그 번호 F170S
제품 가격(KRW)
281,000
Each
카트에 추가하기
반응 수:
250 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
281,000
Each
카트에 추가하기
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
사양
핫 스타트Built-In Hot Start
라벨 또는 염료 포함Yes
반응 수250 Reactions
오버행Blunt
중합효소Phire Hot Start II DNA Polymerase
제품 유형Direct PCR Master Mix
수량250 reactions
반응 형식SuperMix or Master Mix
샘플 종류Tissue
배송 조건Wet or Dry Ice
시작 물질DNA
농도2X
용도(애플리케이션)Genotyping, Hot-start PCR
GC-Rich PCR PerformanceMedium
PCR 방법Direct PCR
반응 속도Fast
Unit SizeEach
구성 및 보관
• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 5 mL
• DNARelease Additive, 3 x 100 μL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.

자주 묻는 질문(FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

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