FluoZin™-3, AM, cell permeant

인용 및 참조 문헌 (43)

Invitrogen™

FluoZin™-3, AM, cell permeant

FluoZin™-3 indicator는 Zn2+ 선택적 표지자로 fluo-4와 유사한 구조를 갖습니다. FluoZin™-3은 1 μM 이상 Ca2+ 농도에 영향을 받지 않는 높은 Zn2+자세히 알아보기

카탈로그 번호	수량
F24195	100 μg

카탈로그 번호 F24195

제품 가격(KRW)

461,000

카트에 추가하기

100 μg

제품 가격(KRW)

461,000

카트에 추가하기

FluoZin™-3 indicator는 Zn²⁺ 선택적 표지자로 fluo-4와 유사한 구조를 갖습니다. FluoZin™-3은 1 μM 이상 Ca²⁺ 농도에 영향을 받지 않는 높은 Zn²⁺ 결합 친화도를 보입니다. 또한, FluoZin™-3은 Zn²⁺ 포화 수준 반응에서 50배 이상의 형광을 나타냅니다. FluoZin™-3의 영상 어플리케이션이 많이 사용되긴 하지만 세포기반 마이크로플레이트 분석과 유세포분석 프로토콜도 개발되어 있습니다. FluoZin™-3 indicator는 세포 투과성 및 세포 비투과성 형태로 이용 가능합니다.

Fluorescent Zinc Indicators 상세:

• 라벨 (Ex/Em): FluoZin™-3 (∼494/516 nm)
• K_d (Zn²⁺) (버퍼에서): ∼15 nM
• 동결건조 제품은 사용 전에 DMSO에 용해해야 할 수 있습니다.
• 제품은 대개 용해된 indicator를 세포 함유 배지에 추가해 세포에 부하합니다.

Zinc 및 기타 금속 이온에 대한 형광 표지자 찾기
본사는 세포 내 polyvalent cation 농도 측정, 이온 경로를 통한 금속 이온 전달 추적, 환경 샘플 측정 등을 위해 많은 형광 표지자를 제공합니다. 이들 제품에 대한 자세한 정보는 Molecular Probes™ Handbook에서 Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7 을 참조하세요. 인간이나 동물의 치료 또는 진단용으로 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.

검출 방법Fluorescence

염료 유형Zinc Indicator

분자량982.85 g/mol

수량100 μg

배송 조건Room Temperature

용도(애플리케이션)Cell Viability and Proliferation

용도 (장비)Fluorescence Microscope

제품라인FluoZin

제품 유형Zinc Indicator

Unit SizeEach

구성 및 보관

Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (43)

인용 및 참조 문헌

Abstract

Demand for Zn2+ in acid-secreting gastric mucosa and its requirement for intracellular Ca2+.

Authors:Liu J, Kohler JE, Blass AL, Moncaster JA, Mocofanescu A, Marcus MA, Blakely EA, Bjornstad KA, Amarasiriwardena C, Casey N, Goldstein LE, Soybel DI,

Journal:PLoS One

PubMed ID:21698273

'Recent work has suggested that Zn(2+) plays a critical role in regulating acidity within the secretory compartments of isolated gastric glands. Here, we investigate the content, distribution and demand for Zn(2+) in gastric mucosa under baseline conditions and its regulation during secretory stimulation. Content and distribution of zinc were evaluated ... More

The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-Nitric Oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo.

Authors:Cappai R, Cheng F, Ciccotosto GD, Needham BE, Masters CL, Multhaup G, Fransson LA, Mani K

Journal:J Biol Chem

PubMed ID:15677459

'Processing of the recycling proteoglycan glypican-1 involves the release of its heparan sulfate chains by copper ion- and nitric oxide-catalyzed ascorbate-triggered autodegradation. The Alzheimer disease amyloid precursor protein (APP) and its paralogue, the amyloid precursor-like protein 2 (APLP2), contain copper ion-, zinc ion-, and heparan sulfate-binding domains. We have investigated ... More

Clioquinol and pyrithione activate TRPA1 by increasing intracellular Zn2+.

Authors:Andersson DA, Gentry C, Moss S, Bevan S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19416844

'The antifungal and amoebicidal drug clioquinol (CQ) was withdrawn from the market when it was linked to an epidemic of subacute myelo-optico-neuropathy (SMON). Clioquinol exerts its anti-parasitic actions by acting as a Cu/Zn chelator and ionophore. Here we show that local injections of CQ produce mechanical hyperalgesia and cold hypersensitivity ... More

Intracellular zinc fluctuations modulate protein tyrosine phosphatase activity in insulin/insulin-like growth factor-1 signaling.

Authors:Haase H, Maret W

Journal:Exp Cell Res

PubMed ID:14644152

'Zinc is an effector of insulin/IGF-1 signaling and has insulinomimetic effects, the molecular basis of which is not understood. The present study establishes the capacity of zinc to inhibit protein tyrosine phosphatases (PTPs) as a cause for these effects and, moreover, demonstrates modulation of the insulin response by changes in ... More

Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response.

Authors:Liuzzi JP, Lichten LA, Rivera S, Blanchard RK, Aydemir TB, Knutson MD, Ganz T, Cousins RJ

Journal:Proc Natl Acad Sci U S A

PubMed ID:15863613

'Infection and inflammation produce systemic responses that include hypozincemia and hypoferremia. The latter involves regulation of the iron transporter ferroportin 1 by hepcidin. The mechanism of reduced plasma zinc is not known. Transcripts of the two zinc transporter gene families (ZnT and Zip) were screened for regulation in mouse liver ... More

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