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인용 및 참조 문헌 (14)
Invitrogen™
Fc OxyBURST™ Green Assay Reagent
Fc OxyBURST™ Green Assay Reagent
| 카탈로그 번호 | 수량 |
|---|---|
| F2902 | 500 μL, 25 reactions |
카탈로그 번호 F2902
제품 가격(KRW)
708,000
Each
수량:
500 μL, 25 reactions
제품 가격(KRW)
708,000
Each
The Fc OxyBURST™ Green assay reagent comprises bovine serum albumin (BSA) that has been covalently linked to dichlorodihydrofluorescein (H2DCF) and then complexed with a purified rabbit polyclonal anti-BSA antibody. When this immune complex binds to Fc receptors, the nonfluorescent H2DCF molecules are internalized within the phagovacuoloe and subsequently oxidized to green-fluorescent 2''7'-dichlorofluorescein.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도3 mg⁄ml
검출 방법Fluorescence
염료 유형Free Radical Sensor
형태Solution
형식Tube(s), Slide(s)
수량500 μL, 25 reactions
배송 조건Wet Ice
용해도DMSO (Dimethylsulfoxide)
Emission488/523
용도 (장비)Fluorescence Microscope, Flow Cytometer
제품라인OXYBURST
제품 유형Assay Reagent
Unit SizeEach
구성 및 보관
Contains 1 vial of Fc OxyBURST™ assay green reagent (suspension in PBS containing sodium azide). Store in refrigerator (2–8°C) and protect from light.
인용 및 참조 문헌 (14)
인용 및 참조 문헌
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without
Fluorescent probes for living cells.
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion