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인용 및 참조 문헌 (22)
Invitrogen™
FluoReporter™ lacZ/Galactosidase Quantitation Kit
The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| F2905 | 1 Kit |
카탈로그 번호 F2905
제품 가격(KRW)
508,000
Each
수량:
1 Kit
제품 가격(KRW)
508,000
Each
The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.
For Research Use Only. Not for use in diagnostic procedures.
사양
어세이β-Gal (lacZ) Assay
검출 방법Fluorescence
제품라인FluoReporter
제품 유형Beta-Galactosidase Reporter Gene Assay System
수량1 Kit
배송 조건Room Temperature
기질CUG
기질 특성Chemical Substrate
기질 유형Beta-Gal Substrate
타겟 효소Beta-Galactosidase
형식Kit
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.
인용 및 참조 문헌 (22)
인용 및 참조 문헌
Abstract
Substrate-labeled fluorescent immunoassay for phenobarbital.
Journal:Ther Drug Monit
PubMed ID:7013165
An assay for the anticonvulsant drug phenobarbital (PB) has been developed that is based on the principles of the substrate-labeled fluorescent immunoassay. A fluorogenic enzyme substrate, galactosyl umbelliferone, was covalently linked to a derivative of PB. The labeled drug, galactosyl umbelliferone-PB (GUPB), is nonfluorescent under conditions of the assay; however,
Substrate-labeled fluorescent immunoassay for measuring dibekacin concentrations in serum and plasma.
Journal:Antimicrob Agents Chemother
PubMed ID:6357071
'We have developed a homogeneous substrate-labeled fluorescent immunoassay for the measurement of dibekacin concentrations in serum and plasma. The fluorogenic enzyme substrate beta-galactosyl-umbelliferone was covalently attached to tobramycin, an aminoglycoside structurally similar to dibekacin, to prepare a fluorogenic drug reagent (FDR). The FDR is nonfluorescent under assay conditions, but fluoresces
A homogeneous fluorescent immunoassay for human immunoglobulin M.
Journal:Clin Chem
PubMed ID:7014030
'We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensity of the labeled IgM at 450 nm (with excitation at 400 nm) is negligible, but when
Substrate-labeled fluorescent immunoassay for phenytoin in human serum.
Journal:Clin Chem
PubMed ID:108033
'A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosidase. When antibody to
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
Journal:J Virol
PubMed ID:12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from