FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain
FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain
인용 및 참조 문헌 (23)
Invitrogen™

FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain

친지질성(lipophilic) probe인 FM™ 4-64FX는 물에서 낮은 형광을 발하나 세포막 외엽에 결합하는 경우에는 높은 형광을 보여 세포막을 염색합니다. 이 결합은 빠르고자세히 알아보기
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카탈로그 번호수량
F3465310 x 100 μg
카탈로그 번호 F34653
제품 가격(KRW)
803,000
Online offer
Ends: 31-Dec-2025
892,000
할인액 89,000 (10%)
Each
카트에 추가하기
수량:
10 x 100 μg
제품 가격(KRW)
803,000
Online offer
Ends: 31-Dec-2025
892,000
할인액 89,000 (10%)
Each
카트에 추가하기
친지질성(lipophilic) probe인 FM™ 4-64FX는 물에서 낮은 형광을 발하나 세포막 외엽에 결합하는 경우에는 높은 형광을 보여 세포막을 염색합니다. 이 결합은 빠르고 가역적이며 'FX'는 이 유사체가 알데히드 기반 고정제로 고정된다는 것을 의미합니다. 이는 또한 활성인 발화 뉴런 확인과 활성 의존적 vesicle cycling 대사의 조사에도 탁월한 reagent입니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Infrared
검출 방법Fluorescence
용도(장비)Fluorescence Microscope
제품라인FM
수량10 x 100 μg
배송 조건Room Temperature
라벨 유형Fluorescent Dye
제품 유형Lipophilic Probe
SubCellular LocalizationPlasma Membrane
Unit SizeEach
구성 및 보관
Store at room temperature and protect from light.

자주 묻는 질문(FAQ)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (23)

인용 및 참조 문헌
Abstract
Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.
Authors:Xiong JP, Mahalingham B, Alonso JL, Borrelli LA, Rui X, Anand S, Hyman BT, Rysiok T, Müller-Pompalla D, Goodman SL, Arnaout MA,
Journal:J Cell Biol
PubMed ID:19704023
'We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and ... More
Endocytic trafficking from the small intestinal brush border probed with FM dye.
Authors:Hansen GH, Rasmussen K, Niels-Christiansen LL, Danielsen EM,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:19679822
The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More
A stochastic mechanism for biofilm formation by Mycoplasma pulmonis.
Authors:Simmons WL, Bolland JR, Daubenspeck JM, Dybvig K,
Journal:J Bacteriol
PubMed ID:17142389
Bacterial biofilms are communities of bacteria that are enclosed in an extracellular matrix. Within a biofilm the bacteria are protected from antimicrobials, environmental stresses, and immune responses from the host. Biofilms are often believed to have a highly developed organization that is derived from differential regulation of the genes that ... More
Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.
Authors:Shen W, Wu B, Zhang Z, Dou Y, Rao ZR, Chen YR, Duan S
Journal:Neuron
PubMed ID:16675395
Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in ... More
Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli.
Authors:Ghosh AS, Young KD
Journal:J Bacteriol
PubMed ID:15743937
In bacteria, several physiological processes once thought to be the products of uniformly dispersed reactions are now known to be highly asymmetric, with some exhibiting interesting geometric localizations. In particular, the cell envelope of Escherichia coli displays a form of subcellular differentiation in which peptidoglycan and outer membrane proteins at ... More
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