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Thermo Scientific™
Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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| 카탈로그 번호 | 화학물질 이름 또는 재질 | 색상 |
|---|---|---|
| F519L | GC Buffer Pack | Colorless |
| F518L | HF Buffer Pack | Colorless |
| F520L | Detergent-free FF Buffer Pack | Colorless |
| F521L | Detergent-free GC Buffer Pack | Colorless |
| F538L | HF Buffer Pack | Green |
| F539L | GC Buffer Pack | Green |
카탈로그 번호 F519L
제품 가격(KRW)
33,000
Online offer
Ends: 31-Dec-2025
38,000할인액 5,000 (13%)
Each
화학물질 이름 또는 재질:
GC Buffer Pack
색상:
Colorless
제품 가격(KRW)
33,000
Online offer
Ends: 31-Dec-2025
38,000할인액 5,000 (13%)
Each
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:
- 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
- 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 이름 또는 재질GC Buffer Pack
색상Colorless
설명Phusion™ GC Buffer Pack
포함• 5X Phusion GC Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)
Store at –20°C.
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)
Store at –20°C.
용도(애플리케이션)Standard PCR
수량4 x 1.5 mL
Unit SizeEach