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Thermo Scientific™
Phire Reaction Buffers
The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents or Green version containing a density reagent and two tracking dyes for direct loading of PCR products on gel.
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| 카탈로그 번호 | 화학물질 이름 또는 재질 |
|---|---|
| F527L | Phire Green Reaction Buffer |
| F524L | Phire Reaction Buffer |
| F525L | Phire Reaction Buffer, detergent-free |
카탈로그 번호 F527L
제품 가격(KRW)
31,000
Online offer
Ends: 31-Dec-2025
36,000할인액 5,000 (14%)
Each
화학물질 이름 또는 재질:
Phire Green Reaction Buffer
제품 가격(KRW)
31,000
Online offer
Ends: 31-Dec-2025
36,000할인액 5,000 (14%)
Each
Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase. This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.
Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm.
The concentration of Mg2+ in Phire Reaction Buffer is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.
Notes
- For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 이름 또는 재질Phire Green Reaction Buffer
설명Green
포함• 5X Phire Green Reaction Buffer, 4 x 1.5 mL
• 50 mM MgCl2, 2 x 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.
• 50 mM MgCl2, 2 x 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.
농도5X
용도(애플리케이션)Hot-start PCR
수량4 x 1.5 mL
Unit SizeEach