Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
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Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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카탈로그 번호포함반응 수
F532SGC Buffer100 Reactions
F532LGC Buffer500 Reactions
F531LHF Buffer500 Reactions
F531SHF Buffer100 Reactions
카탈로그 번호 F532S
제품 가격(KRW)
270,000
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Ends: 31-Dec-2025
317,000
할인액 47,000 (15%)
Each
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포함:
GC Buffer
반응 수:
100 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
270,000
Online offer
Ends: 31-Dec-2025
317,000
할인액 47,000 (15%)
Each
카트에 추가하기
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2. Two master mix formulations are available: with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

  • High fidelity (25X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed

Applications

  • Amplification of difficult (GC-rich) templates
  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR

Using Phusion DNA polymerases

Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)52X
핫 스타트No
포함GC Buffer
반응 수100 Reactions
오버행Blunt
중합효소Phusion High-Fidelity DNA Polymerase
제품 유형High-Fidelity PCR Master Mix
수량100 reactions
반응 형식SuperMix or Master Mix
배송 조건Dry Ice
크기(최종 제품)20 kb or less
농도2X
용도(애플리케이션)Standard PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• 2X Phusion Master Mix with GC Buffer (2 x 1.25 mL)
• 100% DMSO (1 x 500 μL)

Store at –20°C.

자주 묻는 질문(FAQ)

Do Phusion DNA Polymerases add the non-template dependent 3'-A overhang?

Phusion DNA Polymerases generate blunt end products; therefore, blunt end cloning is recommended. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with e.g. Taq DNA Polymerase (Cat. No. EP0401). However, before adding the overhangs it is very important to remove all the Phusion DNA Polymerase by purifying the PCR product carefully, as the proofreading activity in Phusion DNA Polymerase is very strong. Any remaining Phusion DNA Polymerase will degrade the A overhangs, thus creating blunt ends again.

Can Phusion DNA Polymerases extend at 1 second/kb?

Yes it is possible, especially when amplifying smaller amplicons. Processivity of Phusion DNA Polymerases is 10 times that of Pfu. We recommend extension times of 15 s/kb for Phusion Flash PCR Master Mix. 15 s/kb is a conservative value that we can promise to work with almost any amplicon. In many cases, significantly shorter extension times (0-5 s/kb) can be used without compromising the yield. What separates Phusion Flash DNA Polymerase from other fast polymerases is that all steps in the PCR protocol can be shortened, including annealing and denaturation. This results in extremely fast protocols as compared with any other polymerase.