Search

  • 주문 현황
  • 빠른주문
Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호색상반응 수
F534SGreen100 Reactions
F530SColorless100 Reactions
F530LColorless500 Reactions
F-530XLColorless2000 Reactions
F534LGreen500 Reactions
카탈로그 번호 F534S
제품 가격(KRW)
177,000
Online offer
Ends: 31-Mar-2026
202,000
할인액 25,000 (12%)
Each
카트에 추가하기
색상:
Green
반응 수:
100 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
177,000
Online offer
Ends: 31-Mar-2026
202,000
할인액 25,000 (12%)
Each
카트에 추가하기
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

For Research Use Only. Not for use in diagnostic procedures.
사양
색상Green
Fidelity (Taq 대비)52X
핫 스타트No
반응 수100 Reactions
오버행Blunt
중합효소Phusion High-Fidelity DNA Polymerase
제품 유형High-Fidelity DNA Polymerase
수량100 units
반응 형식Standalone
배송 조건Dry Ice
크기(최종 제품)20 kb or less
농도2 U/μL
용도(애플리케이션)Standard PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• Phusion DNA Polymerase, 2 U/μL
• 5X Phusion Green HF and GF Buffers
• DMSO
• 50 mM MgCl2

Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.

자주 묻는 질문(FAQ)

Do Phusion DNA Polymerases add the non-template dependent 3'-A overhang?

Phusion DNA Polymerases generate blunt end products; therefore, blunt end cloning is recommended. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with e.g. Taq DNA Polymerase (Cat. No. EP0401). However, before adding the overhangs it is very important to remove all the Phusion DNA Polymerase by purifying the PCR product carefully, as the proofreading activity in Phusion DNA Polymerase is very strong. Any remaining Phusion DNA Polymerase will degrade the A overhangs, thus creating blunt ends again.

Can Phusion DNA Polymerases extend at 1 second/kb?

Yes it is possible, especially when amplifying smaller amplicons. Processivity of Phusion DNA Polymerases is 10 times that of Pfu. We recommend extension times of 15 s/kb for Phusion Flash PCR Master Mix. 15 s/kb is a conservative value that we can promise to work with almost any amplicon. In many cases, significantly shorter extension times (0-5 s/kb) can be used without compromising the yield. What separates Phusion Flash DNA Polymerase from other fast polymerases is that all steps in the PCR protocol can be shortened, including annealing and denaturation. This results in extremely fast protocols as compared with any other polymerase.

Can protocols optimized for Phusion DNA Polymerase be directly applied to Phusion Hot Start II DNA Polymerase?

Yes, protocols optimized for Phusion DNA Polymerase can be applied to Phusion Hot Start II DNA Polymerase reactions.

What nucleotide analogues can I use with DyNAzyme and Phusion DNA Polymerases?

DyNAzyme II DNA Polymerase can use dUTP, biotinylated dNTPs, 7-deaza-dGTP, digoxigenin-dUTP, bromo-dUTP, radiolabeled dNTPs and ITP. DyNAzyme EXT DNA Polymerase and Phusion DNA Polymerase cannot read dUTP-derivatives or dITP in the template strand so the use of these analogues is not recommended. Use Phusion U Hot Start DNA Polymerase for amplification of dUTP and dITP containing templates.

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.

View all Phusion™ High-Fidelity DNA Polymerases (2 U/μL) FAQs