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Thermo Scientific™
Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).
| 카탈로그 번호 | 수량 |
|---|---|
| F549N | 2000 reactions |
카탈로그 번호 F549N
제품 가격(KRW)
3,355,000
온라인 행사
Ends: 31-Dec-2025
3,946,000할인액 591,000 (15%)
Each
수량:
2000 reactions
제품 가격(KRW)
3,355,000
온라인 행사
Ends: 31-Dec-2025
3,946,000할인액 591,000 (15%)
Each
Thermo Scientific Phusion Hot Start II High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq and 6-fold lower than that of Pfu, this polymerase is an excellent choice for cloning and other applications that require high fidelity and offers robust performance with short protocol times, even in the presence of PCR inhibitors. Phusion Hot Start II High-Fidelity DNA Polymerase generates higher yields with lower enzyme amounts than other DNA polymerases.
With Phusion Hot Start II High-Fidelity DNA Polymerase, amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3' to 5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction setup. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II High-Fidelity DNA Polymerase is immediately reactivated at high temperatures and does not require a separate activation step in PCR protocols.
Features
- High fidelity (52X Taq)
- No non-specific amplification and primer degradation during reaction setup
- Fast PCR due to short extension times (15–30 s/kb)
- Increased product yields with minimal enzyme amounts
Applications
- Standard PCR
- qPCR
- High fidelity and long PCR
- LAMP-PCR
- cDNA synthesis
- RT-PCR, RDA, MDA
- DNA labeling and sequencing
dNTP Mix
Thermo Scientific dNTP Mix (Cat. No. R0192) contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.
- Greater than 99% purity confirmed by HPLC
- Free of human and E. coli DNA
- Stable after multiple freeze-thaw cycles
- Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
- Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)
Notes
- This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high-quality dNTP Mix (10 mM each, 2 x 1 mL).
- Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)52X
핫 스타트Built-In Hot Start
반응 수2000 Reactions
오버행Blunt
중합효소Phusion Hot Start II DNA Polymerase
제품 유형Hot Start DNA Polymerase and dNTP Mix
수량2000 reactions
반응 형식Separate Components
배송 조건Dry Ice
크기(최종 제품)20 kb or less
농도2 U/μL
검출 방법Primer-probe
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도Fast
Unit SizeEach
구성 및 보관
• Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U at 2 U/μL)
• 10 mM dNTP Mix (2 x 1 mL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Store at –5°C to –30°C.
• 10 mM dNTP Mix (2 x 1 mL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Store at –5°C to –30°C.