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Thermo Scientific™
Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
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보기 방식 변경
| 카탈로그 번호 | 반응 수 |
|---|---|
| F630XL | 2000 Reactions |
| F630L | 500 Reactions |
| F630S | 100 Reactions |
카탈로그 번호 F630XL
제품 가격(KRW)
2,719,000
Online offer
Ends: 31-Mar-2026
3,107,000할인액 388,000 (12%)
Each
반응 수:
2000 Reactions
제품 가격(KRW)
2,719,000
Online offer
Ends: 31-Mar-2026
3,107,000할인액 388,000 (12%)
Each
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.
Features include:
- >100X higher sequence accuracy than Taq polymerase
- Universal annealing temperature of 60°C in PCR
- Hot-start mechanism reduces non-specific amplification and primer degradation
- Automation enabled due to 24-hour stability of assembled reactions
- High inhibitor tolerance for work with DNA of suboptimal purity
The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.
Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.
Applications
- High-fidelity PCR
- Cloning & mutagenesis
- Template generation for sequencing
- GC-rich PCR
- Long-range PCR (up to 20 kb)
- High-throughput PCR
Notes
- To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.
For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)>100X
핫 스타트Built-In Hot Start
반응 수2000 Reactions
오버행Blunt
중합효소Phusion Plus DNA Polymerase
수량2000 x 50 μL reactions
반응 형식Separate components
배송 조건Room Temp or Wet Ice
크기(최종 제품)20 kb or less
용도(애플리케이션)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
반응 속도15 to 30 sec./kb
Unit SizeEach
구성 및 보관
Four packs, each containing:
• Phusion Plus DNA Polymerase (250 μL)
• 5X Phusion Plus Buffer (5 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
Store at –15°C to –25°C.
• Phusion Plus DNA Polymerase (250 μL)
• 5X Phusion Plus Buffer (5 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
Store at –15°C to –25°C.