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Thermo Scientific™
Bovine Genotypes Panel 2.2 Kit
Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR)자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 반응 수 |
|---|---|
| F847S | 100 Reactions |
| F847L | 500 Reactions |
카탈로그 번호 F847S
제품 가격(KRW)
1,138,000
Each
반응 수:
100 Reactions
제품 가격(KRW)
1,138,000
Each
Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.
The Bovine Genotypes Panel 2.2 allows co-amplification of the 6 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 2.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'.
Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.
The Bovine Genotypes Panel 2.2 is intended to be used as a complement to the Bovine Genotypes Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes Panel 1.2 kit (see Figure 2).
Highlights
• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)
This product has been developed in collaboration with Maxxam Analytics Inc.
The Bovine Genotypes Panel 2.2 allows co-amplification of the 6 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 2.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'.
Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.
The Bovine Genotypes Panel 2.2 is intended to be used as a complement to the Bovine Genotypes Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes Panel 1.2 kit (see Figure 2).
Highlights
• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)
This product has been developed in collaboration with Maxxam Analytics Inc.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Capillary Electrophoresis
용도(애플리케이션)PCR
반응 수100 Reactions
제품 유형Bovine Genotypes Panel 2.2 Kit
수량100 reactions
타겟Bovine Genotyping and Parentage Testing
기술STR (Short Tandem Repeat) Analysis
Unit SizeEach
구성 및 보관
Contains:
• 1.1 mL Bovine Genotypes Master Mix
• 1.1 mL Bovine Genotypes Panel 2.2 Primer Mix
• 30 μL Bovine Genotypes Control DNA001
Store at -20°C. Primer Mix should be protected from light at all the times.
• 1.1 mL Bovine Genotypes Master Mix
• 1.1 mL Bovine Genotypes Panel 2.2 Primer Mix
• 30 μL Bovine Genotypes Control DNA001
Store at -20°C. Primer Mix should be protected from light at all the times.