FluoSpheres™ Carboxylate-Modified Microspheres

인용 및 참조 문헌 (22)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8803
Price: 607000 KRW

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.

보기 방식 변경

카탈로그 번호	직경(미터법)	색상	여기/방출	수량
F8803	0.1 μm	Yellow-Green	505/515 nm	10 mL
F8816	1.0 μm	Crimson	625/645 nm	2 mL
F8823	1.0 μm	Yellow-Green	505/515 nm	10 mL
F8801	0.1 μm	Red	580/605 nm	10 mL
F8811	0.2 μm	Yellow-Green	505/515 nm	10 mL
F8807	0.2 μm	Dark Red	660/680 nm	2 mL
F10720	0.04 μm	Yellow-Green, Orange, Red, Dark Red	505/515, 540/560, 580/605, 660/680 nm	1 mL/each
F20881	0.2 μm	Orange	365/610 nm	2 mL
F8783 F-8783으로도 사용됨	0.02 μm	Dark Red	660/680 nm	2 mL
F8786	0.02 μm	Red	580/605 nm	10 mL
F8795 F-8795으로도 사용됨	0.04 μm	Yellow-Green	505/515 nm	1 mL
F8813	0.5 μm	Yellow-Green	505/515 nm	10 mL
F8820	1.0 μm	Orange	540/560 nm	10 mL
F8825	2.0 μm	Nile Red	535/575 nm	2 mL
F8827	2.0 μm	Yellow-Green	505/515 nm	2 mL
F8781 F-8781으로도 사용됨	0.02 μm	Blue	365/415 nm	10 mL
F8782 F-8782으로도 사용됨	0.02 μm	Crimson	625/645 nm	2 mL
F8784	0.02 μm	Nile Red	535/575 nm	10 mL
F8787	0.02 μm	Yellow-Green	505/515 nm	10 mL
F8789 F-8789으로도 사용됨	0.04 μm	Dark Red	660/680 nm	1 mL
F8792 F-8792으로도 사용됨	0.04 μm	Orange	540/560 nm	1 mL
F8793	0.04 μm	Red	580/605 nm	1 mL
F8794	0.04 μm	Red-Orange	565/580 nm	1 mL
F8797	0.1 μm	Blue	350/440 nm	10 mL
F8799	0.1 μm	Infrared	715/755 nm	1 mL
F8800 F-8800으로도 사용됨	0.1 μm	Orange	540/560 nm	10 mL
F8805	0.2 μm	Blue	365/415 nm	10 mL
F8806	0.2 μm	Crimson	625/645 nm	2 mL
F8809	0.2 μm	Orange	540/560 nm	10 mL
F8810	0.2 μm	Red	580/605 nm	10 mL
F8812	0.5 μm	Red	580/605 nm	10 mL
F8814	1.0 μm	Blue	365/415 nm	10 mL
F8815	1.0 μm	Blue	350/440 nm	10 mL
F8819	1.0 μm	Nile Red	535/575 nm	10 mL
F8821	1.0 μm	Red	580/605 nm	10 mL
F8824 F-8824으로도 사용됨	2.0 μm	Blue	365/415 nm	2 mL
F8826	2.0 μm	Red	580/605 nm	2 mL

37 개 옵션

카탈로그 번호 F8803

제품 가격(KRW)

607,000

온라인 행사

Ends: 31-Mar-2026

713,000

할인액 106,000 (15%)

Each

카트에 추가하기

직경(미터법):

0.1 μm

색상:

Yellow-Green

여기/방출:

505/515 nm

수량:

10 mL

제품 가격(KRW)

607,000

온라인 행사

Ends: 31-Mar-2026

713,000

할인액 106,000 (15%)

Each

카트에 추가하기

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.

For Research Use Only. Not for use in diagnostic procedures.

사양

여기/방출505/515 nm

제품라인FLUOSPHERES

수량10 mL

표면 수정Carboxylate

색상Yellow-Green

직경(미터법)0.1 μm

용도(애플리케이션)Fluorescence Microscopy

물질Polystyrene

제품 유형Carboxylate-Modified Microsphere

Unit SizeEach

구성 및 보관

Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

인용 및 참조 문헌 (22)

인용 및 참조 문헌

Abstract

Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.

Authors:Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW,

Journal:Biophys J

PubMed ID:18326650

'Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resolution, with true ... More

The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.

Authors:Niesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,

Journal:Biophys J

PubMed ID:17557785

'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More

Role of glycocalyx in leukocyte-endothelial cell adhesion.

Authors:Mulivor AW, Lipowsky HH

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:12234777

'The binding of fluorescently labeled microspheres (FLMs, 0.1-microm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-microm length of venule after superfusion of the ... More

a2 Adrenergic receptor-mediated inhibition of thermogenesis.

Authors:Madden CJ, Tupone D, Cano G, Morrison SF,

Journal:J Neurosci

PubMed ID:23365239

'a2 adrenergic receptor (a2-AR) agonists have been used as antihypertensive agents, in the management of drug withdrawal, and as sedative analgesics. Since a2-AR agonists also influence the regulation of body temperature, we explored their potential as antipyretic agents. This study delineates the central neural substrate for the inhibition of rat ... More

Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.

Authors:Madden CJ,

Journal:Am J Physiol Regul Integr Comp Physiol

PubMed ID:22071154

'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More

View all FluoSpheres™ Carboxylate-Modified Microspheres citations

FluoSpheres™ Carboxylate-Modified Microspheres

자주 묻는 질문(FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

인용 및 참조 문헌 (22)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?