FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
인용 및 참조 문헌 (4)
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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카탈로그 번호직경(미터법)색상여기/방출수량
F88262.0 μmRed580/605 nm2 mL
F88030.1 μmYellow-Green505/515 nm10 mL
F88161.0 μmCrimson625/645 nm2 mL
F88231.0 μmYellow-Green505/515 nm10 mL
F88010.1 μmRed580/605 nm10 mL
F88110.2 μmYellow-Green505/515 nm10 mL
F88070.2 μmDark Red660/680 nm2 mL
F107200.04 μmYellow-Green, Orange, Red, Dark Red505/515, 540/560, 580/605, 660/680 nm1 mL/each
F208810.2 μmOrange365/610 nm2 mL
F8783
F-8783으로도 사용됨
0.02 μmDark Red660/680 nm2 mL
F87860.02 μmRed580/605 nm10 mL
F8795
F-8795으로도 사용됨
0.04 μmYellow-Green505/515 nm1 mL
F88130.5 μmYellow-Green505/515 nm10 mL
F88201.0 μmOrange540/560 nm10 mL
F88252.0 μmNile Red535/575 nm2 mL
F88272.0 μmYellow-Green505/515 nm2 mL
F8781
F-8781으로도 사용됨
0.02 μmBlue365/415 nm10 mL
F8782
F-8782으로도 사용됨
0.02 μmCrimson625/645 nm2 mL
F87840.02 μmNile Red535/575 nm10 mL
F87870.02 μmYellow-Green505/515 nm10 mL
F8789
F-8789으로도 사용됨
0.04 μmDark Red660/680 nm1 mL
F8792
F-8792으로도 사용됨
0.04 μmOrange540/560 nm1 mL
F87930.04 μmRed580/605 nm1 mL
F87940.04 μmRed-Orange565/580 nm1 mL
F87970.1 μmBlue350/440 nm10 mL
F87990.1 μmInfrared715/755 nm1 mL
F8800
F-8800으로도 사용됨
0.1 μmOrange540/560 nm10 mL
F88050.2 μmBlue365/415 nm10 mL
F88060.2 μmCrimson625/645 nm2 mL
F88090.2 μmOrange540/560 nm10 mL
F88100.2 μmRed580/605 nm10 mL
F88120.5 μmRed580/605 nm10 mL
F88141.0 μmBlue365/415 nm10 mL
F88151.0 μmBlue350/440 nm10 mL
F88191.0 μmNile Red535/575 nm10 mL
F88211.0 μmRed580/605 nm10 mL
F8824
F-8824으로도 사용됨
2.0 μmBlue365/415 nm2 mL
카탈로그 번호 F8826
제품 가격(KRW)
553,000
Online offer
Ends: 31-Dec-2025
691,000
할인액 138,000 (20%)
Each
카트에 추가하기
직경(미터법):
2.0 μm
색상:
Red
여기/방출:
580/605 nm
수량:
2 mL
제품 가격(KRW)
553,000
Online offer
Ends: 31-Dec-2025
691,000
할인액 138,000 (20%)
Each
카트에 추가하기

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
사양
여기/방출580/605 nm
제품라인FLUOSPHERES
수량2 mL
표면 수정Carboxylate
색상Red
직경(미터법)2.0 μm
용도(애플리케이션)Fluorescence Microscopy
물질Polystyrene
제품 유형Carboxylate-Modified Microsphere
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long and at what concentration should I sonicate my FluoSpheres Carboxylate-Modified Microspheres (Cat. No. F8826) before using them?

You can rock the particles gently to resuspend and then sonicate in a bath type sonicator for about 5 minutes. Particles can be sonicated at any concentration, but we recommend users just sonicate the stock bottle.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌
Abstract
Tuftsin binds neuropilin-1 through a sequence similar to that encoded by exon 8 of vascular endothelial growth factor.
Authors:von Wronski MA,Raju N,Pillai R,Bogdan NJ,Marinelli ER,Nanjappan P,Ramalingam K,Arunachalam T,Eaton S,Linder KE,Yan F,Pochon S,Tweedle MF,Nunn AD
Journal:The Journal of biological chemistry
PubMed ID:16371354
Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones.
Authors:Trueta C, Méndez B, De-Miguel FF
Journal:J Physiol
PubMed ID:12562971
'We studied somatic exocytosis of serotonin and its mediation by L-type calcium (Ca2+) channels in cultured Retzius neurones of the leech. Exocytosis was induced by trains of impulses at different frequencies or by depolarisation with 40 mM potassium (K+), and was quantified by use of the fluorescent dye FM 1-43. ... More
Light transmission technique for the evaluation of colloidal transport and dynamics in porous media.
Authors:Weisbrod N, Niemet MR, Selker JS
Journal:Environ Sci Technol
PubMed ID:12953883
Colloidal transport in porous media has been typically studied in column experiments from which data analysis was limited to the evaluation of effluent breakthrough curves and/or destructive sampling at the end of the experiments. The internal processes occur within a "black box", where direct observation is not possible and therefore ... More
Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors.
Authors:O'Brien DK, Melville SB
Journal:Microbiology
PubMed ID:12777479
Clostridium perfringens is a Gram-positive, anaerobic bacterium that is the most common cause of gas gangrene (clostridial myonecrosis) in humans. C. perfringens produces a variety of extracellular toxins that are thought to be the major virulence factors of the organism. However, C. perfringens has recently been shown to have the ... More