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인용 및 참조 문헌 (8)
Invitrogen™
Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| G12411 | 100 μL |
카탈로그 번호 G12411
제품 가격(KRW)
458,000
Online offer
Ends: 31-Dec-2025
538,000할인액 80,000 (15%)
Each
수량:
100 μL
제품 가격(KRW)
458,000
Online offer
Ends: 31-Dec-2025
538,000할인액 80,000 (15%)
Each
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
For Research Use Only. Not for use in diagnostic procedures.
사양
라벨 또는 염료BODIPY™ FL
제품 유형Guanosine 5'-Triphosphate
수량100 μL
배송 조건Wet Ice
농도5 mM
제품라인BODIPY
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.
인용 및 참조 문헌 (8)
인용 및 참조 문헌
Abstract
Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.
Journal:Anal Chem
PubMed ID:17263349
'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product,
Automated single-cell electroporation.
Journal:Biotechniques
PubMed ID:17068953
Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.
Journal:J Biomol Screen
PubMed ID:20008126
Small GTPases are key regulators of cellular activity and represent novel targets for the treatment of human diseases using small-molecule inhibitors. The authors describe a multiplex, flow cytometry bead-based assay for the identification and characterization of inhibitors or activators of small GTPases. Six different glutathione-S-transferase (GST)-tagged small GTPases were bound
A direct fluorescence-based assay for RGS domain GTPase accelerating activity.
Journal:Anal Biochem
PubMed ID:15840508
Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide binding proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the Galpha subunit. RGS (regulator of
Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.
Journal:J Biol Chem
PubMed ID:15613467
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max