FIX & PERM™ Cell Permeabilization Kit
FIX & PERM™ Cell Permeabilization Kit
인용 및 참조 문헌 (2)
Invitrogen™

FIX & PERM™ Cell Permeabilization Kit

The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing자세히 알아보기
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카탈로그 번호테스트 수
GAS00350 Tests
GAS004200 Tests
카탈로그 번호 GAS003
제품 가격(KRW)
244,000
線上優惠
Ends: 31-Mar-2026
287,000
할인액 43,000 (15%)
Each
카트에 추가하기
테스트 수:
50 Tests
제품 가격(KRW)
244,000
線上優惠
Ends: 31-Mar-2026
287,000
할인액 43,000 (15%)
Each
카트에 추가하기
The FIX & PERM™ Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.



• Flexible—compatible with multiple sample types and cellular antigens

• Robust—scatter pattern of cells unaffected

• Efficient workflow—simultaneous detection of intracellular and surface markers



The FIX & PERM™ Cell Permeabilization Kit is suitable for various biological samples and has been validated for use with cell lines, whole blood, and bone marrow. The FIX & PERM™ kit has been shown to accurately identify previously undetectable intracellular markers. Unlike other fixation and permeabilization reagents available on the market, the FIX & PERM™ staining protocol leaves the morphological scatter characteristics of the cells intact.



The FIX & PERM™ formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.



How It Works:

The FIX & PERM™ Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Reagent A) and Permeabilization Medium (Reagent B). Cells are incubated for 15 minutes at room temperature with the fixation medium, washed, and then incubated with the permeabilization medium and fluorochrome-labeled antibody.



The FIX & PERM™ Cell Permeabilization Kit is available in two sizes (50 and 200 tests). Reagent A and Reagent B are also available separately in a bulk size (1000 tests).
For Research Use Only. Not for use in diagnostic procedures.
사양
테스트 수50 Tests
제품라인FIX & PERM
수량50 tests
배송 조건Room Temperature
제품 유형Fixation/Permeabilization Reagent
Unit SizeEach

자주 묻는 질문(FAQ)

Is the FIX & PERM Cell Permeabilization Kit (Cat. No. GAS003, GAS004) compatible with conjugates (dyes) besides FITC and PE?

Yes, this product is compatible with other conjugated antibodies, outside of FITC and PE.

The FIX & PERM Cell Permeabilization Kit protocol does not say what volume my cells should be in when they are added to the tube. What should I do?

Your cells should be in approximately 100 µL volume. One way to do this is to aliquot the correct number of cells (10E6) and then spin them down and decant the supernatant. The residual volume should be approximately 100 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the FIX & PERM Cell Permeabilization Kit to access nuclear epitopes?

Yes. Generally, you need to increase the incubation time in the permeabilization buffer (Reagent B). Here are a couple of references:
-Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320-2330(http://www.ncbi.nlm.nih.gov/pubmed/9799760).
-Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2-related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932-3938(http://www.ncbi.nlm.nih.gov/pubmed/11090080).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The FIX & PERM Cell Permeabilization Kit is used to prepare cells for flow cytometry; can I use it for immunohistochemistry?

Yes. Here are a couple of references:
Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753-7759 (http://www.ncbi.nlm.nih.gov/pubmed/11705957).

Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624-1628 (http://www.ncbi.nlm.nih.gov/pubmed/10875878).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the FIX & PERM Cell Permeabilization Kit (Cat. Nos. GAS003 and GAS004) lyse red blood cells?

Yes. The FIX & PERM Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics. It has been validated for use in cell lines, whole blood, and bone marrow. In addition fixing and permeabilizing cells, it lyses red blood cells. It is labeled as a general purpose reagent (GPR) and can be used in clinical settings, but it is also used widely in research labs.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Roles of Pofut1 and O-fucose in mammalian Notch signaling.
Authors:Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P,
Journal:J Biol Chem
PubMed ID:18347015
Mammalian Notch receptors contain 29-36 epidermal growth factor (EGF)-like repeats that may be modified by protein O-fucosyltransferase 1 (Pofut1), an essential component of the canonical Notch signaling pathway. The Drosophila orthologue Ofut1 is proposed to function as both a chaperone required for stable cell surface expression of Notch and a ... More
Synaptotagmin-mediated vesicle fusion regulates cell migration.
Authors:Colvin RA, Means TK, Diefenbach TJ, Moita LF, Friday RP, Sever S, Campanella GS, Abrazinski T, Manice LA, Moita C, Andrews NW, Wu D, Hacohen N, Luster AD,
Journal:Nat Immunol
PubMed ID:20473299
Chemokines and other chemoattractants direct leukocyte migration and are essential for the development and delivery of immune and inflammatory responses. To probe the molecular mechanisms that underlie chemoattractant-guided migration, we did an RNA-mediated interference screen that identified several members of the synaptotagmin family of calcium-sensing vesicle-fusion proteins as mediators of ... More