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인용 및 참조 문헌 (4)
Invitrogen™
ImaGene Red™ C12RG lacZ Gene Expression Kit
The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| I2906 | 1 kit |
카탈로그 번호 I2906
제품 가격(KRW)
1,086,000
Each
수량:
1 kit
제품 가격(KRW)
1,086,000
Each
The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
For Research Use Only. Not for use in diagnostic procedures.
사양
어세이β-Gal (lacZ) Assay
검출 방법Fluorescence
제품라인DetectaGene
제품 유형β-Galactosidase Reporter Gene Assay System
수량1 kit
배송 조건Room Temperature
기질C12RG
기질 특성Chemical Substrate
기질 유형Beta-Gal Substrate
타겟 효소Beta-Galactosidase
형식Kit
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.
인용 및 참조 문헌 (4)
인용 및 참조 문헌
Abstract
Vital staining of cardiac myocytes during embryonic stem cell cardiogenesis in vitro.
Journal:Circ Res
PubMed ID:8635211
'Mouse embryonic stem (ES) cells differentiate in vitro into a variety of cell types, including spontaneously contracting cardiac myocytes. The primary aim of this work was to use vital stain techniques for real-time detection of developing cardiac myocytes in ES cell differentiation cultures. The -440 to +6 human cardiac alpha-actin
Activity of Hb9 interneurons during fictive locomotion in mouse spinal cord.
Journal:J Neurosci
PubMed ID:19759307
'Hb9 interneurons (Hb9 INs) are putative components of the mouse spinal locomotor central pattern generator (CPG) and candidates for the rhythm-generating kernel. Studies in slices and hemisected spinal cords showed that Hb9 INs display TTX-resistant membrane potential oscillations, suggesting a role in rhythm generation. To further investigate the roles of
Functional ephrin-B2 expression for promotive interaction between arterial and venous vessels in postnatal neovascularization.
Journal:Circulation
PubMed ID:15851594
'Ephrin-B2, one of the transmembrane ligands, is a genetic marker of arterial endothelial cells (ECs) at embryonic stages and is essential for cardiovascular development, but its roles in ischemic cardiovascular disease are not well understood. In this study, we focused on the function of ephrin-B2 in postnatal neovascularization. We found
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14976247
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use for functional cellular studies remains hampered by the lack of single-cell assays for hemocytes (blood cells). Here we introduce a generic method combining fluorescence-activated cell sorting and nonantibody probes that enables the selective gating