PCR Master Mix (2X)
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PCR Master Mix (2X)
Thermo Scientific™

PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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카탈로그 번호반응 수
K0171200 Reactions
K01721000 Reactions
카탈로그 번호 K0171
제품 가격(KRW)
138,000
온라인 행사
Ends: 31-Dec-2025
153,000
할인액 15,000 (10%)
Each
카트에 추가하기
반응 수:
200 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
138,000
온라인 행사
Ends: 31-Dec-2025
153,000
할인액 15,000 (10%)
Each
카트에 추가하기
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min. at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Note

The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

For Research Use Only. Not for use in diagnostic procedures.
사양
Fidelity (Taq 대비)1X
핫 스타트No
반응 수200 Reactions
오버행3'-A
중합효소Taq DNA Polymerase
제품 유형PCR Master Mix
수량200 reactions
반응 형식SuperMix or Master Mix
시작 물질DNA
농도2X
용도(애플리케이션)Standard PCR
GC-Rich PCR PerformanceLow
반응 속도Standard
Unit SizeEach
구성 및 보관
• 2X PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -20°C.

자주 묻는 질문(FAQ)

Can I do PCR directly from bacterial colonies with Thermo Scientific Taq Master Mixes?

Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.