Luminaris Color Probe qPCR Master Mix
Product Image
Thermo Scientific™

Luminaris Color Probe qPCR Master Mix

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호반응 수
K0351250 Reactions
K03531,250 Reactions
K0352500 reactions
카탈로그 번호 K0351
제품 가격(KRW)
405,000
Online offer
Ends: 30-Jun-2026
449,000
할인액 44,000 (10%)
Each
카트에 추가하기
반응 수:
250 Reactions
제품 가격(KRW)
405,000
Online offer
Ends: 30-Jun-2026
449,000
할인액 44,000 (10%)
Each
카트에 추가하기
**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.
사양
설명Color Probe
용도(장비)BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
형식Tube
반응 수250 Reactions
중합효소Taq DNA Polymerase
제품라인Luminaris
제품 유형qPCR Master Mix
수량250 x 20 μL Reactions
샘플 종류DNA, dsDNA
충분250 Reactions of 20 μL
부피2 x 1.25 mL
농도2X (qPCR Master Mix), 40X (Buffer)
검출 방법Primer-probe
용도(애플리케이션)Gene Expression
GC-Rich PCR PerformanceHigh
PCR 방법qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Contains:
• 2 x 1.25 mL Luminaris Color Probe qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer with a blue dye; sufficient for 250 x 20 μL reactions
• 1 x 1.25 mL 40X Yellow Sample Buffer
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

자주 묻는 질문(FAQ)

Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?

Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.

What enzyme is used in Luminaris qPCR Master Mixes?

Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.

Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?

When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.