CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells.자세히 알아보기

카탈로그 번호	수량
K1023	5 mg

카탈로그 번호 K1023

제품 가격(KRW)

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.

For Research Use Only. Not for use in diagnostic procedures.

세포 투과성Cell-Permeant

용도(장비)Flow Cytometer, Microplate Reader

제품라인GeneBLAzer

수량5 mg

배송 조건Room Temperature

기질CCF2-AM

검출 방법Fluorescence

Substrate PropertiesChemical Substrate

기질 유형Beta-Lactamase Substrate

Unit SizeEach

구성 및 보관

Store in freezer (-5 to -30°C).

자주 묻는 질문(FAQ)

Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

Folate receptor alpha and caveolae are not required for Ebola virus glycoprotein-mediated viral infection.

Authors:Simmons G, Rennekamp AJ, Chai N, Vandenberghe LH, Riley JL, Bates P,

Journal:J Virol

PubMed ID:14645601

Folate receptor alpha (FRalpha) has been described as a factor involved in mediating Ebola virus entry into cells (6). Furthermore, it was suggested that interaction with FRalpha results in internalization and subsequent viral ingress into the cytoplasm via caveolae (9). Descriptions of cellular receptors for Ebola virus and its entry ... More